Genomic analysis of the origins and evolution of multicentric diffuse lower-grade gliomas

Josie Hayes, Yao Yu, Llewellyn E. Jalbert, Tali Mazor, Lindsey E. Jones, Matthew Wood, Kyle M. Walsh, Henrik Bengtsson, Chibo Hong, Stefan Oberndorfer, Thomas Roetzer, Ivan V. Smirnov, Jennifer L. Clarke, Manish K. Aghi, Susan M. Chang, Sarah J. Nelson, Adelheid Woehrer, Joanna J. Phillips, David A. Solomon, Joseph F. Costello

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background Rare multicentric lower-grade gliomas (LGGs) represent a unique opportunity to study the heterogeneity among distinct tumor foci in a single patient and to infer their origins and parallel patterns of evolution. Methods In this study, we integrate clinical features, histology, and immunohistochemistry for 4 patients with multicentric LGG, arising both synchronously and metachronously. For 3 patients we analyze the phylogeny of the lesions using exome sequencing, including one case with a total of 8 samples from the 2 lesions. Results One patient was diagnosed with multicentric isocitrate dehydrogenase 1 (IDH1) mutated diffuse astrocytomas harboring distinct IDH1 mutations, R132H and R132C; the latter mutation has been associated with Li-Fraumeni syndrome, which was subsequently confirmed in the patient's germline DNA and shown in additional cases with The Cancer Genome Atlas data. In another patient, phylogenetic analysis of synchronously arising grade II and grade III diffuse astrocytomas demonstrated a single shared mutation, IDH1 R132H, and revealed convergent evolution via non-overlapping mutations in ATRX and TP53. In 2 cases, there was divergent evolution of IDH1-mutated and 1p/19q-codeleted oligodendroglioma and IDH1-mutated and 1p/19q-intact diffuse astrocytoma, occurring synchronously in one case and metachronously in a second. Conclusions Each tumor in multicentric LGG cases may arise independently or may diverge very early in their development, presenting as genetically and histologically distinct tumors. Comprehensive sampling of these lesions can therefore significantly alter diagnosis and management. Additionally, somatic IDH1 R132C mutation in either multicentric or solitary LGG identifies unsuspected germline TP53 mutation, validating the limited number of published cases.

Original languageEnglish (US)
Pages (from-to)632-641
Number of pages10
JournalNeuro-Oncology
Volume20
Issue number5
DOIs
StatePublished - Apr 9 2018
Externally publishedYes

Fingerprint

Isocitrate Dehydrogenase
Glioma
Astrocytoma
Mutation
Neoplasms
Li-Fraumeni Syndrome
Exome
Oligodendroglioma
Germ-Line Mutation
Atlases
Phylogeny
Histology
Immunohistochemistry
Genome
DNA

Keywords

  • astrocytoma
  • bilateral
  • IDH1
  • multicentric
  • oligodendroglioma

ASJC Scopus subject areas

  • Oncology
  • Clinical Neurology
  • Cancer Research

Cite this

Hayes, J., Yu, Y., Jalbert, L. E., Mazor, T., Jones, L. E., Wood, M., ... Costello, J. F. (2018). Genomic analysis of the origins and evolution of multicentric diffuse lower-grade gliomas. Neuro-Oncology, 20(5), 632-641. https://doi.org/10.1093/neuonc/nox205

Genomic analysis of the origins and evolution of multicentric diffuse lower-grade gliomas. / Hayes, Josie; Yu, Yao; Jalbert, Llewellyn E.; Mazor, Tali; Jones, Lindsey E.; Wood, Matthew; Walsh, Kyle M.; Bengtsson, Henrik; Hong, Chibo; Oberndorfer, Stefan; Roetzer, Thomas; Smirnov, Ivan V.; Clarke, Jennifer L.; Aghi, Manish K.; Chang, Susan M.; Nelson, Sarah J.; Woehrer, Adelheid; Phillips, Joanna J.; Solomon, David A.; Costello, Joseph F.

In: Neuro-Oncology, Vol. 20, No. 5, 09.04.2018, p. 632-641.

Research output: Contribution to journalArticle

Hayes, J, Yu, Y, Jalbert, LE, Mazor, T, Jones, LE, Wood, M, Walsh, KM, Bengtsson, H, Hong, C, Oberndorfer, S, Roetzer, T, Smirnov, IV, Clarke, JL, Aghi, MK, Chang, SM, Nelson, SJ, Woehrer, A, Phillips, JJ, Solomon, DA & Costello, JF 2018, 'Genomic analysis of the origins and evolution of multicentric diffuse lower-grade gliomas', Neuro-Oncology, vol. 20, no. 5, pp. 632-641. https://doi.org/10.1093/neuonc/nox205
Hayes, Josie ; Yu, Yao ; Jalbert, Llewellyn E. ; Mazor, Tali ; Jones, Lindsey E. ; Wood, Matthew ; Walsh, Kyle M. ; Bengtsson, Henrik ; Hong, Chibo ; Oberndorfer, Stefan ; Roetzer, Thomas ; Smirnov, Ivan V. ; Clarke, Jennifer L. ; Aghi, Manish K. ; Chang, Susan M. ; Nelson, Sarah J. ; Woehrer, Adelheid ; Phillips, Joanna J. ; Solomon, David A. ; Costello, Joseph F. / Genomic analysis of the origins and evolution of multicentric diffuse lower-grade gliomas. In: Neuro-Oncology. 2018 ; Vol. 20, No. 5. pp. 632-641.
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abstract = "Background Rare multicentric lower-grade gliomas (LGGs) represent a unique opportunity to study the heterogeneity among distinct tumor foci in a single patient and to infer their origins and parallel patterns of evolution. Methods In this study, we integrate clinical features, histology, and immunohistochemistry for 4 patients with multicentric LGG, arising both synchronously and metachronously. For 3 patients we analyze the phylogeny of the lesions using exome sequencing, including one case with a total of 8 samples from the 2 lesions. Results One patient was diagnosed with multicentric isocitrate dehydrogenase 1 (IDH1) mutated diffuse astrocytomas harboring distinct IDH1 mutations, R132H and R132C; the latter mutation has been associated with Li-Fraumeni syndrome, which was subsequently confirmed in the patient's germline DNA and shown in additional cases with The Cancer Genome Atlas data. In another patient, phylogenetic analysis of synchronously arising grade II and grade III diffuse astrocytomas demonstrated a single shared mutation, IDH1 R132H, and revealed convergent evolution via non-overlapping mutations in ATRX and TP53. In 2 cases, there was divergent evolution of IDH1-mutated and 1p/19q-codeleted oligodendroglioma and IDH1-mutated and 1p/19q-intact diffuse astrocytoma, occurring synchronously in one case and metachronously in a second. Conclusions Each tumor in multicentric LGG cases may arise independently or may diverge very early in their development, presenting as genetically and histologically distinct tumors. Comprehensive sampling of these lesions can therefore significantly alter diagnosis and management. Additionally, somatic IDH1 R132C mutation in either multicentric or solitary LGG identifies unsuspected germline TP53 mutation, validating the limited number of published cases.",
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AU - Yu, Yao

AU - Jalbert, Llewellyn E.

AU - Mazor, Tali

AU - Jones, Lindsey E.

AU - Wood, Matthew

AU - Walsh, Kyle M.

AU - Bengtsson, Henrik

AU - Hong, Chibo

AU - Oberndorfer, Stefan

AU - Roetzer, Thomas

AU - Smirnov, Ivan V.

AU - Clarke, Jennifer L.

AU - Aghi, Manish K.

AU - Chang, Susan M.

AU - Nelson, Sarah J.

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AU - Phillips, Joanna J.

AU - Solomon, David A.

AU - Costello, Joseph F.

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N2 - Background Rare multicentric lower-grade gliomas (LGGs) represent a unique opportunity to study the heterogeneity among distinct tumor foci in a single patient and to infer their origins and parallel patterns of evolution. Methods In this study, we integrate clinical features, histology, and immunohistochemistry for 4 patients with multicentric LGG, arising both synchronously and metachronously. For 3 patients we analyze the phylogeny of the lesions using exome sequencing, including one case with a total of 8 samples from the 2 lesions. Results One patient was diagnosed with multicentric isocitrate dehydrogenase 1 (IDH1) mutated diffuse astrocytomas harboring distinct IDH1 mutations, R132H and R132C; the latter mutation has been associated with Li-Fraumeni syndrome, which was subsequently confirmed in the patient's germline DNA and shown in additional cases with The Cancer Genome Atlas data. In another patient, phylogenetic analysis of synchronously arising grade II and grade III diffuse astrocytomas demonstrated a single shared mutation, IDH1 R132H, and revealed convergent evolution via non-overlapping mutations in ATRX and TP53. In 2 cases, there was divergent evolution of IDH1-mutated and 1p/19q-codeleted oligodendroglioma and IDH1-mutated and 1p/19q-intact diffuse astrocytoma, occurring synchronously in one case and metachronously in a second. Conclusions Each tumor in multicentric LGG cases may arise independently or may diverge very early in their development, presenting as genetically and histologically distinct tumors. Comprehensive sampling of these lesions can therefore significantly alter diagnosis and management. Additionally, somatic IDH1 R132C mutation in either multicentric or solitary LGG identifies unsuspected germline TP53 mutation, validating the limited number of published cases.

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