Genome-wide analysis of the Drosophila immune response by using oligonucleotide microarrays

Ennio De Gregorio, Paul T. Spellman, Gerald M. Rubin, Bruno Lemaitre

Research output: Contribution to journalArticle

548 Scopus citations

Abstract

To identify new Drosophila genes involved in the immune response, we monitored the gene expression profile of adult flies in response to microbial infection by using high-density oligonucleotide microarrays encompassing nearly the full Drosophila genome. Of 13,197 genes tested, we have characterized 230 induced and 170 repressed by microbial infection, most of which had not previously been associated with the immune response. Many of these genes can be assigned to specific aspects of the immune response, including recognition, phagocytosis, coagulation, melanization, activation of NF-KB transcription factors, synthesis of antimicrobial peptides, production of reactive oxygen species, and regulation of iron metabolism. Additionally, we found a large number of genes with unknown function that may be involved in control and execution of the immune response. Determining the function of these genes represents an important challenge for improving our knowledge of innate immunity. Complete results may be found at http://www.fruitfly.org/expression/immunity/.

Original languageEnglish (US)
Pages (from-to)12590-12595
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number22
DOIs
StatePublished - Oct 23 2001
Externally publishedYes

Keywords

  • Fungal infection
  • Innate immunity
  • Septic injury

ASJC Scopus subject areas

  • General

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