TY - JOUR
T1 - Genome-wide analysis of the Drosophila immune response by using oligonucleotide microarrays
AU - De Gregorio, Ennio
AU - Spellman, Paul T.
AU - Rubin, Gerald M.
AU - Lemaitre, Bruno
PY - 2001/10/23
Y1 - 2001/10/23
N2 - To identify new Drosophila genes involved in the immune response, we monitored the gene expression profile of adult flies in response to microbial infection by using high-density oligonucleotide microarrays encompassing nearly the full Drosophila genome. Of 13,197 genes tested, we have characterized 230 induced and 170 repressed by microbial infection, most of which had not previously been associated with the immune response. Many of these genes can be assigned to specific aspects of the immune response, including recognition, phagocytosis, coagulation, melanization, activation of NF-KB transcription factors, synthesis of antimicrobial peptides, production of reactive oxygen species, and regulation of iron metabolism. Additionally, we found a large number of genes with unknown function that may be involved in control and execution of the immune response. Determining the function of these genes represents an important challenge for improving our knowledge of innate immunity. Complete results may be found at http://www.fruitfly.org/expression/immunity/.
AB - To identify new Drosophila genes involved in the immune response, we monitored the gene expression profile of adult flies in response to microbial infection by using high-density oligonucleotide microarrays encompassing nearly the full Drosophila genome. Of 13,197 genes tested, we have characterized 230 induced and 170 repressed by microbial infection, most of which had not previously been associated with the immune response. Many of these genes can be assigned to specific aspects of the immune response, including recognition, phagocytosis, coagulation, melanization, activation of NF-KB transcription factors, synthesis of antimicrobial peptides, production of reactive oxygen species, and regulation of iron metabolism. Additionally, we found a large number of genes with unknown function that may be involved in control and execution of the immune response. Determining the function of these genes represents an important challenge for improving our knowledge of innate immunity. Complete results may be found at http://www.fruitfly.org/expression/immunity/.
KW - Fungal infection
KW - Innate immunity
KW - Septic injury
UR - http://www.scopus.com/inward/record.url?scp=0035940514&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035940514&partnerID=8YFLogxK
U2 - 10.1073/pnas.221458698
DO - 10.1073/pnas.221458698
M3 - Article
C2 - 11606746
AN - SCOPUS:0035940514
SN - 0027-8424
VL - 98
SP - 12590
EP - 12595
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 22
ER -