TY - JOUR
T1 - Genetic screen reveals link between the maternal effect sterile gene mes-1 and pseudomonas aeruginosa-induced neurodegeneration in caenorhabditis elegans
AU - Wu, Qiuli
AU - Cao, Xiou
AU - Yan, Dong
AU - Wang, Dayong
AU - Aballay, Alejandro
N1 - Publisher Copyright:
© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.
PY - 2015/12/4
Y1 - 2015/12/4
N2 - Increasing evidence indicates that immune responses to microbial infections may contribute to neurodegenerative diseases. Here, we show that Pseudomonas aeruginosa infection of Caenorhabditis elegans causes a number of neural changes that are hallmarks of neurodegeneration. Using an unbiased genetic screen to identify genes involved in the control of P. aeruginosainduced neurodegeneration, we identified mes-1, which encodes a receptor tyrosine kinase-like protein that is required for unequal cell divisions in the early embryonic germ line. We showed that sterile but not fertile mes-1 animals were resistant to neurodegeneration induced by P. aeruginosa infection. Similar results were observed using animals carrying a mutation in the maternal effect gene PGL-1, which is required for postembryonic germ line development, and the germ line-deficient strains glp-1 and glp-4. Additional studies indicated that the FOXO transcription factor DAF-16 is required for resistance to P. aeruginosa-induced neurodegeneration in germ line-deficient strains. Thus, our results demonstrate that P. aeruginosa infection results in neurodegeneration phenotypes in C. Elegans that are controlled by the germ line in a cell-nonautonomous manner.
AB - Increasing evidence indicates that immune responses to microbial infections may contribute to neurodegenerative diseases. Here, we show that Pseudomonas aeruginosa infection of Caenorhabditis elegans causes a number of neural changes that are hallmarks of neurodegeneration. Using an unbiased genetic screen to identify genes involved in the control of P. aeruginosainduced neurodegeneration, we identified mes-1, which encodes a receptor tyrosine kinase-like protein that is required for unequal cell divisions in the early embryonic germ line. We showed that sterile but not fertile mes-1 animals were resistant to neurodegeneration induced by P. aeruginosa infection. Similar results were observed using animals carrying a mutation in the maternal effect gene PGL-1, which is required for postembryonic germ line development, and the germ line-deficient strains glp-1 and glp-4. Additional studies indicated that the FOXO transcription factor DAF-16 is required for resistance to P. aeruginosa-induced neurodegeneration in germ line-deficient strains. Thus, our results demonstrate that P. aeruginosa infection results in neurodegeneration phenotypes in C. Elegans that are controlled by the germ line in a cell-nonautonomous manner.
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U2 - 10.1074/jbc.M115.674259
DO - 10.1074/jbc.M115.674259
M3 - Article
C2 - 26475858
AN - SCOPUS:84946084426
SN - 0021-9258
VL - 290
SP - 29231
EP - 29239
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 49
ER -