Genetic demonstration of bidirectionality in the high affinity purine base transporter of mutant mouse S49 cells.

J. T. Beck, Buddy Ullman

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A mutant cell line was selected from wild type S49 lymphoblasts that expressed a novel high affinity purine base transport system not found in parental cells or any other mammalian cell line (Aronow, B., Toll, D., Patrick, J., Hollingsworth, P., McCartan, K., and Ullman, B. (1986) Mol. Cell. Biol. 6, 2957-2962). In order to determine whether this nucleobase transport system was bidirectional, mutant cell lines possessing this high affinity base transport capability were derived from a nucleoside transport-deficient derivative of an adenylosuccinate synthetase-deficient S49 cell line. The resulting progeny excreted significantly greater amounts of purine into the cell culture medium than parental cells. This purine was identified as hypoxanthine. These results demonstrate genetically that the high affinity purine base transport system can mediate both the influx and efflux of hypoxanthine.

Original languageEnglish (US)
Pages (from-to)2393-2397
Number of pages5
JournalJournal of Biological Chemistry
Volume262
Issue number5
StatePublished - Feb 15 1987
Externally publishedYes

Fingerprint

Demonstrations
Cells
Cell Line
Hypoxanthine
Adenylosuccinate Synthase
Nucleosides
Cell culture
Culture Media
Cell Culture Techniques
Derivatives
purine

ASJC Scopus subject areas

  • Biochemistry

Cite this

Genetic demonstration of bidirectionality in the high affinity purine base transporter of mutant mouse S49 cells. / Beck, J. T.; Ullman, Buddy.

In: Journal of Biological Chemistry, Vol. 262, No. 5, 15.02.1987, p. 2393-2397.

Research output: Contribution to journalArticle

@article{4ef552acf9fd4619a7e3f653fee8c22e,
title = "Genetic demonstration of bidirectionality in the high affinity purine base transporter of mutant mouse S49 cells.",
abstract = "A mutant cell line was selected from wild type S49 lymphoblasts that expressed a novel high affinity purine base transport system not found in parental cells or any other mammalian cell line (Aronow, B., Toll, D., Patrick, J., Hollingsworth, P., McCartan, K., and Ullman, B. (1986) Mol. Cell. Biol. 6, 2957-2962). In order to determine whether this nucleobase transport system was bidirectional, mutant cell lines possessing this high affinity base transport capability were derived from a nucleoside transport-deficient derivative of an adenylosuccinate synthetase-deficient S49 cell line. The resulting progeny excreted significantly greater amounts of purine into the cell culture medium than parental cells. This purine was identified as hypoxanthine. These results demonstrate genetically that the high affinity purine base transport system can mediate both the influx and efflux of hypoxanthine.",
author = "Beck, {J. T.} and Buddy Ullman",
year = "1987",
month = "2",
day = "15",
language = "English (US)",
volume = "262",
pages = "2393--2397",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "5",

}

TY - JOUR

T1 - Genetic demonstration of bidirectionality in the high affinity purine base transporter of mutant mouse S49 cells.

AU - Beck, J. T.

AU - Ullman, Buddy

PY - 1987/2/15

Y1 - 1987/2/15

N2 - A mutant cell line was selected from wild type S49 lymphoblasts that expressed a novel high affinity purine base transport system not found in parental cells or any other mammalian cell line (Aronow, B., Toll, D., Patrick, J., Hollingsworth, P., McCartan, K., and Ullman, B. (1986) Mol. Cell. Biol. 6, 2957-2962). In order to determine whether this nucleobase transport system was bidirectional, mutant cell lines possessing this high affinity base transport capability were derived from a nucleoside transport-deficient derivative of an adenylosuccinate synthetase-deficient S49 cell line. The resulting progeny excreted significantly greater amounts of purine into the cell culture medium than parental cells. This purine was identified as hypoxanthine. These results demonstrate genetically that the high affinity purine base transport system can mediate both the influx and efflux of hypoxanthine.

AB - A mutant cell line was selected from wild type S49 lymphoblasts that expressed a novel high affinity purine base transport system not found in parental cells or any other mammalian cell line (Aronow, B., Toll, D., Patrick, J., Hollingsworth, P., McCartan, K., and Ullman, B. (1986) Mol. Cell. Biol. 6, 2957-2962). In order to determine whether this nucleobase transport system was bidirectional, mutant cell lines possessing this high affinity base transport capability were derived from a nucleoside transport-deficient derivative of an adenylosuccinate synthetase-deficient S49 cell line. The resulting progeny excreted significantly greater amounts of purine into the cell culture medium than parental cells. This purine was identified as hypoxanthine. These results demonstrate genetically that the high affinity purine base transport system can mediate both the influx and efflux of hypoxanthine.

UR - http://www.scopus.com/inward/record.url?scp=0023654049&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023654049&partnerID=8YFLogxK

M3 - Article

C2 - 3029078

AN - SCOPUS:0023654049

VL - 262

SP - 2393

EP - 2397

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 5

ER -