Genes expressed by the biological control bacterium Pseudomonas protegens Pf-5 on seed surfaces under the control of the global regulators GacA and RpoS

Teresa A. Kidarsa, Brenda T. Shaffer, Neal C. Goebel, Daniel P. Roberts, Jeffrey S. Buyer, Aaron Johnson, Donald Y. Kobayashi, Mark Zabriskie, Ian Paulsen, Joyce E. Loper

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Summary: Gene expression profiles of the biological control strain Pseudomonas protegens Pf-5 inhabiting pea seed surfaces were revealed using a whole-genome oligonucleotide microarray. We identified genes expressed by Pf-5 under the control of two global regulators (GacA and RpoS) known to influence biological control and secondary metabolism. Transcript levels of 897 genes, including many with unknown functions as well as those for biofilm formation, cyclic diguanylate (c-di-GMP) signalling, iron homeostasis and secondary metabolism, were influenced by one or both regulators, providing evidence for expression of these genes by Pf-5 on seed surfaces. Comparison of the GacA and RpoS transcriptomes defined for Pf-5 grown on seed versus in broth culture overlapped, but most genes were regulated by GacA or RpoS under only one condition, likely due to differing levels of expression in the two conditions. We quantified secondary metabolites produced by Pf-5 and gacA and rpoS mutants on seed and in culture, and found that production profiles corresponded generally with biosynthetic gene expression profiles. Future studies evaluating biological control mechanisms can now focus on genes expressed by Pf-5 on seed surfaces, the habitat where the bacterium interacts with seed-infecting pathogens to suppress seedling diseases.

Original languageEnglish (US)
Pages (from-to)716-735
Number of pages20
JournalEnvironmental Microbiology
Volume15
Issue number3
DOIs
StatePublished - Mar 2013
Externally publishedYes

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Pseudomonas
biological control
Seeds
Bacteria
seed
bacterium
gene
bacteria
seeds
Transcriptome
Secondary Metabolism
Genes
genes
gene expression
metabolism
seedling diseases
Peas
secondary metabolite
homeostasis
Biofilms

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Genes expressed by the biological control bacterium Pseudomonas protegens Pf-5 on seed surfaces under the control of the global regulators GacA and RpoS. / Kidarsa, Teresa A.; Shaffer, Brenda T.; Goebel, Neal C.; Roberts, Daniel P.; Buyer, Jeffrey S.; Johnson, Aaron; Kobayashi, Donald Y.; Zabriskie, Mark; Paulsen, Ian; Loper, Joyce E.

In: Environmental Microbiology, Vol. 15, No. 3, 03.2013, p. 716-735.

Research output: Contribution to journalArticle

Kidarsa, Teresa A. ; Shaffer, Brenda T. ; Goebel, Neal C. ; Roberts, Daniel P. ; Buyer, Jeffrey S. ; Johnson, Aaron ; Kobayashi, Donald Y. ; Zabriskie, Mark ; Paulsen, Ian ; Loper, Joyce E. / Genes expressed by the biological control bacterium Pseudomonas protegens Pf-5 on seed surfaces under the control of the global regulators GacA and RpoS. In: Environmental Microbiology. 2013 ; Vol. 15, No. 3. pp. 716-735.
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