Generation of high curvature membranes mediated by direct endophilin bilayer interactions

Khashayar Farsad, Niels Ringstad, Kohji Takei, Scott R. Floyd, Kristin Rose, Pietro De Camilli

Research output: Contribution to journalArticle

442 Citations (Scopus)

Abstract

Endophilin 1 is a presynaptically enriched protein which binds the GTPase dynamin and the polyphos-phoinositide phosphatase synptojanin. Perturbation of endophilin function in cell-free systems and in a living synapse has implicated endophilin in endocytic vesicle budding (Ringstad, N., H. Gad, P. Low, G. Di Paolo, L. Brodin, O. Shupliakov, and P. De Camilli. 1999. Neuron. 24:143-154; Schmidt, A., M. Wolde, C. Thiele, W. Fest, H. Kratzin, A.V. Podtelejnikov, W. Witke, W.B. Huttner, and H.D. Soling. 1999. Nature. 401:133-141; Gad, H., N. Ringstad, P. Low, O. Kjaerulff, J. Gustafsson, M. Wenk, G. Di Paolo, Y. Nemoto, J. Crun, M.H. Ellisman, et al. 2000. Neuron. 27:301 -312). Here, we show that purified endophilin can directly bind and evaginate lipid bilayers into narrow tubules similar in diameter to the neck of a clathrin-coated bud, providing new insight into the mechanisms through which endophilin may participate in membrane deformation and vesicle budding. This property of endophilin is independent of its putative lysophosphatydic acid acyl transferase activity, is mediated by its NH2-terminal region, and requires an amino acid stretch homologous to a corresponding region in amphiphysin, a protein previously shown to have similar effects on lipid bilayers (Takei, K., V.I. Slepnev, V. Haucke, and P. De Camilli. 1999. Nat. Cell Biol. 1:33-39). Endophilin cooligomerizes with dynamin rings on lipid tubules and inhibits dynamin's GTP-dependent vesiculating activity. Endophilin B, a protein with homology to endophilin 1, partially localizes to the Golgi complex and also deforms lipid bilayers into tubules, underscoring a potential role of endophilin family members in diverse tubulovesicular membrane-trafficking events in the cell.

Original languageEnglish (US)
Pages (from-to)193-200
Number of pages8
JournalJournal of Cell Biology
Volume155
Issue number2
DOIs
StatePublished - Oct 15 2001
Externally publishedYes

Fingerprint

Dynamins
Lipid Bilayers
Membranes
Neurons
Transport Vesicles
Clathrin
Cell-Free System
GTP Phosphohydrolases
Golgi Apparatus
Transferases
Guanosine Triphosphate
Phosphoric Monoester Hydrolases
Synapses
Proteins
Lipids
Amino Acids
Acids

Keywords

  • Dynamin
  • Endocytosis
  • Endophilin B1
  • RICH
  • SH3P4

ASJC Scopus subject areas

  • Cell Biology

Cite this

Generation of high curvature membranes mediated by direct endophilin bilayer interactions. / Farsad, Khashayar; Ringstad, Niels; Takei, Kohji; Floyd, Scott R.; Rose, Kristin; De Camilli, Pietro.

In: Journal of Cell Biology, Vol. 155, No. 2, 15.10.2001, p. 193-200.

Research output: Contribution to journalArticle

Farsad, Khashayar ; Ringstad, Niels ; Takei, Kohji ; Floyd, Scott R. ; Rose, Kristin ; De Camilli, Pietro. / Generation of high curvature membranes mediated by direct endophilin bilayer interactions. In: Journal of Cell Biology. 2001 ; Vol. 155, No. 2. pp. 193-200.
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