Gene expression-based classification of nonseminomatous male germ cell tumors

James Korkola, Jane Houldsworth, Debbie Dobrzynski, Adam B. Olshen, Victor E. Reuter, George J. Bosl, R. S K Chaganti

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Male adult germ cell tumors (GCTs) comprise two major histologic groups: seminomas and nonseminomas. Nonseminomatous GCTs (NSGCTs) can be further divided into embryonal carcinoma (EC), teratoma (T), yolk sac tumor (YS), and choriocarcinoma (CC) on the basis of the lineage differentiation that they exhibit. NSGCTs frequently present as mixed tumors consisting of two or more histological subtypes, often limiting correlative studies of clinical and molecular features to histology. We sought to develop a molecular classifier that could predict the predominant histologic subtype within mixed NSGCT tumor samples. The expression profiles of 84 NSGCTs (42 pure and 42 mixed) and normal age-matched testes were obtained using Affymetrix microarrays. Using prediction analysis for microarrays, we identified 146 transcripts that classified the histology of pure NSGCTs samples with 93% accuracy. When applied to mixed NSGCTs, the classifier predicted a histology that was consistent with one of the reported components in 93% of cases. Among the predictive transcripts were CGB (high in CC), LCN2 (high in T), BMP2 (high in YS), and POU5F1 (high in EC). Thus, the expression-based classifier accurately assigned a single predominant histology to mixed NSGCTs, and identified transcripts differentially expressed between histologic components with relevance to NSGCT differentiation.

Original languageEnglish (US)
Pages (from-to)5101-5107
Number of pages7
JournalOncogene
Volume24
Issue number32
DOIs
StatePublished - Jul 28 2005
Externally publishedYes

Fingerprint

Histology
Embryonal Carcinoma
Endodermal Sinus Tumor
Gene Expression
Choriocarcinoma
Seminoma
Teratoma
Microarray Analysis
Testis
Neoplasms
Male Germ Cell Tumor
Nonseminomatous germ cell tumor

Keywords

  • Expression microarray
  • Histologic classification
  • Testicular germ cell tumors

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Korkola, J., Houldsworth, J., Dobrzynski, D., Olshen, A. B., Reuter, V. E., Bosl, G. J., & Chaganti, R. S. K. (2005). Gene expression-based classification of nonseminomatous male germ cell tumors. Oncogene, 24(32), 5101-5107. https://doi.org/10.1038/sj.onc.1208694

Gene expression-based classification of nonseminomatous male germ cell tumors. / Korkola, James; Houldsworth, Jane; Dobrzynski, Debbie; Olshen, Adam B.; Reuter, Victor E.; Bosl, George J.; Chaganti, R. S K.

In: Oncogene, Vol. 24, No. 32, 28.07.2005, p. 5101-5107.

Research output: Contribution to journalArticle

Korkola, J, Houldsworth, J, Dobrzynski, D, Olshen, AB, Reuter, VE, Bosl, GJ & Chaganti, RSK 2005, 'Gene expression-based classification of nonseminomatous male germ cell tumors', Oncogene, vol. 24, no. 32, pp. 5101-5107. https://doi.org/10.1038/sj.onc.1208694
Korkola J, Houldsworth J, Dobrzynski D, Olshen AB, Reuter VE, Bosl GJ et al. Gene expression-based classification of nonseminomatous male germ cell tumors. Oncogene. 2005 Jul 28;24(32):5101-5107. https://doi.org/10.1038/sj.onc.1208694
Korkola, James ; Houldsworth, Jane ; Dobrzynski, Debbie ; Olshen, Adam B. ; Reuter, Victor E. ; Bosl, George J. ; Chaganti, R. S K. / Gene expression-based classification of nonseminomatous male germ cell tumors. In: Oncogene. 2005 ; Vol. 24, No. 32. pp. 5101-5107.
@article{4d7108b3a77449738a0860cbef75bca6,
title = "Gene expression-based classification of nonseminomatous male germ cell tumors",
abstract = "Male adult germ cell tumors (GCTs) comprise two major histologic groups: seminomas and nonseminomas. Nonseminomatous GCTs (NSGCTs) can be further divided into embryonal carcinoma (EC), teratoma (T), yolk sac tumor (YS), and choriocarcinoma (CC) on the basis of the lineage differentiation that they exhibit. NSGCTs frequently present as mixed tumors consisting of two or more histological subtypes, often limiting correlative studies of clinical and molecular features to histology. We sought to develop a molecular classifier that could predict the predominant histologic subtype within mixed NSGCT tumor samples. The expression profiles of 84 NSGCTs (42 pure and 42 mixed) and normal age-matched testes were obtained using Affymetrix microarrays. Using prediction analysis for microarrays, we identified 146 transcripts that classified the histology of pure NSGCTs samples with 93{\%} accuracy. When applied to mixed NSGCTs, the classifier predicted a histology that was consistent with one of the reported components in 93{\%} of cases. Among the predictive transcripts were CGB (high in CC), LCN2 (high in T), BMP2 (high in YS), and POU5F1 (high in EC). Thus, the expression-based classifier accurately assigned a single predominant histology to mixed NSGCTs, and identified transcripts differentially expressed between histologic components with relevance to NSGCT differentiation.",
keywords = "Expression microarray, Histologic classification, Testicular germ cell tumors",
author = "James Korkola and Jane Houldsworth and Debbie Dobrzynski and Olshen, {Adam B.} and Reuter, {Victor E.} and Bosl, {George J.} and Chaganti, {R. S K}",
year = "2005",
month = "7",
day = "28",
doi = "10.1038/sj.onc.1208694",
language = "English (US)",
volume = "24",
pages = "5101--5107",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "32",

}

TY - JOUR

T1 - Gene expression-based classification of nonseminomatous male germ cell tumors

AU - Korkola, James

AU - Houldsworth, Jane

AU - Dobrzynski, Debbie

AU - Olshen, Adam B.

AU - Reuter, Victor E.

AU - Bosl, George J.

AU - Chaganti, R. S K

PY - 2005/7/28

Y1 - 2005/7/28

N2 - Male adult germ cell tumors (GCTs) comprise two major histologic groups: seminomas and nonseminomas. Nonseminomatous GCTs (NSGCTs) can be further divided into embryonal carcinoma (EC), teratoma (T), yolk sac tumor (YS), and choriocarcinoma (CC) on the basis of the lineage differentiation that they exhibit. NSGCTs frequently present as mixed tumors consisting of two or more histological subtypes, often limiting correlative studies of clinical and molecular features to histology. We sought to develop a molecular classifier that could predict the predominant histologic subtype within mixed NSGCT tumor samples. The expression profiles of 84 NSGCTs (42 pure and 42 mixed) and normal age-matched testes were obtained using Affymetrix microarrays. Using prediction analysis for microarrays, we identified 146 transcripts that classified the histology of pure NSGCTs samples with 93% accuracy. When applied to mixed NSGCTs, the classifier predicted a histology that was consistent with one of the reported components in 93% of cases. Among the predictive transcripts were CGB (high in CC), LCN2 (high in T), BMP2 (high in YS), and POU5F1 (high in EC). Thus, the expression-based classifier accurately assigned a single predominant histology to mixed NSGCTs, and identified transcripts differentially expressed between histologic components with relevance to NSGCT differentiation.

AB - Male adult germ cell tumors (GCTs) comprise two major histologic groups: seminomas and nonseminomas. Nonseminomatous GCTs (NSGCTs) can be further divided into embryonal carcinoma (EC), teratoma (T), yolk sac tumor (YS), and choriocarcinoma (CC) on the basis of the lineage differentiation that they exhibit. NSGCTs frequently present as mixed tumors consisting of two or more histological subtypes, often limiting correlative studies of clinical and molecular features to histology. We sought to develop a molecular classifier that could predict the predominant histologic subtype within mixed NSGCT tumor samples. The expression profiles of 84 NSGCTs (42 pure and 42 mixed) and normal age-matched testes were obtained using Affymetrix microarrays. Using prediction analysis for microarrays, we identified 146 transcripts that classified the histology of pure NSGCTs samples with 93% accuracy. When applied to mixed NSGCTs, the classifier predicted a histology that was consistent with one of the reported components in 93% of cases. Among the predictive transcripts were CGB (high in CC), LCN2 (high in T), BMP2 (high in YS), and POU5F1 (high in EC). Thus, the expression-based classifier accurately assigned a single predominant histology to mixed NSGCTs, and identified transcripts differentially expressed between histologic components with relevance to NSGCT differentiation.

KW - Expression microarray

KW - Histologic classification

KW - Testicular germ cell tumors

UR - http://www.scopus.com/inward/record.url?scp=23744511288&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23744511288&partnerID=8YFLogxK

U2 - 10.1038/sj.onc.1208694

DO - 10.1038/sj.onc.1208694

M3 - Article

VL - 24

SP - 5101

EP - 5107

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 32

ER -