Gangliosides GM1 and GD1b are not polarized in mature hippocampal neurons

Hidehiko Shogomori; Michelle A. Burack; Gary Banker; Anthony H. Futerman

doi:10.1016/S0014-5793(99)01146-1

Gangliosides GM1 and GD1b are not polarized in mature hippocampal neurons

Hidehiko Shogomori, Michelle A. Burack, Gary Banker, Anthony H. Futerman

Neurology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Analysis of the binding of cholera toxin to ganglioside GM1 in both living and fixed neurons, and comparison with the distribution of defined axonal and dendritic proteins, demonstrates that ganglioside GM1 is distributed in a non-polarized manner over the axonal and dendritic plasma membranes of mature, cultured hippocampal neurons. Likewise, ganglioside GD1b is also distributed in a non-polarized manner. These results suggest that a recent report [Ledesma, M.D. et al. EMBO J. 18 (1999) 1761-1771] proposing that ganglioside GM1 is highly enriched on the axonal versus dendritic membrane of hippocampal neurons may need to be re-evaluated. Copyright (C) 1999 Federation of European Biochemical Societies.

Original language	English (US)
Pages (from-to)	107-111
Number of pages	5
Journal	FEBS Letters
Volume	458
Issue number	2
DOIs	https://doi.org/10.1016/S0014-5793(99)01146-1
State	Published - Sep 17 1999

Keywords

Axon
Cholera toxin
Dendrite
Ganglioside
Neuron
Polarity

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(99)01146-1

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title = "Gangliosides GM1 and GD1b are not polarized in mature hippocampal neurons",

abstract = "Analysis of the binding of cholera toxin to ganglioside GM1 in both living and fixed neurons, and comparison with the distribution of defined axonal and dendritic proteins, demonstrates that ganglioside GM1 is distributed in a non-polarized manner over the axonal and dendritic plasma membranes of mature, cultured hippocampal neurons. Likewise, ganglioside GD1b is also distributed in a non-polarized manner. These results suggest that a recent report [Ledesma, M.D. et al. EMBO J. 18 (1999) 1761-1771] proposing that ganglioside GM1 is highly enriched on the axonal versus dendritic membrane of hippocampal neurons may need to be re-evaluated. Copyright (C) 1999 Federation of European Biochemical Societies.",

keywords = "Axon, Cholera toxin, Dendrite, Ganglioside, Neuron, Polarity",

author = "Hidehiko Shogomori and Burack, {Michelle A.} and Gary Banker and Futerman, {Anthony H.}",

note = "Funding Information: This work was supported by Grant 95-254 from the US-Israel Binational Science Foundation. We thank Rivi Zisling (Rehovot) and Jon Muyskens (Portland) for help in preparing and maintaining the hippocampal cultures.",

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T1 - Gangliosides GM1 and GD1b are not polarized in mature hippocampal neurons

AU - Shogomori, Hidehiko

AU - Burack, Michelle A.

AU - Banker, Gary

AU - Futerman, Anthony H.

N1 - Funding Information: This work was supported by Grant 95-254 from the US-Israel Binational Science Foundation. We thank Rivi Zisling (Rehovot) and Jon Muyskens (Portland) for help in preparing and maintaining the hippocampal cultures.

PY - 1999/9/17

Y1 - 1999/9/17

N2 - Analysis of the binding of cholera toxin to ganglioside GM1 in both living and fixed neurons, and comparison with the distribution of defined axonal and dendritic proteins, demonstrates that ganglioside GM1 is distributed in a non-polarized manner over the axonal and dendritic plasma membranes of mature, cultured hippocampal neurons. Likewise, ganglioside GD1b is also distributed in a non-polarized manner. These results suggest that a recent report [Ledesma, M.D. et al. EMBO J. 18 (1999) 1761-1771] proposing that ganglioside GM1 is highly enriched on the axonal versus dendritic membrane of hippocampal neurons may need to be re-evaluated. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - Analysis of the binding of cholera toxin to ganglioside GM1 in both living and fixed neurons, and comparison with the distribution of defined axonal and dendritic proteins, demonstrates that ganglioside GM1 is distributed in a non-polarized manner over the axonal and dendritic plasma membranes of mature, cultured hippocampal neurons. Likewise, ganglioside GD1b is also distributed in a non-polarized manner. These results suggest that a recent report [Ledesma, M.D. et al. EMBO J. 18 (1999) 1761-1771] proposing that ganglioside GM1 is highly enriched on the axonal versus dendritic membrane of hippocampal neurons may need to be re-evaluated. Copyright (C) 1999 Federation of European Biochemical Societies.

KW - Axon

KW - Cholera toxin

KW - Dendrite

KW - Ganglioside

KW - Neuron

KW - Polarity

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SN - 0014-5793

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JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Gangliosides GM1 and GD1b are not polarized in mature hippocampal neurons

Abstract

Keywords

ASJC Scopus subject areas

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