Further purification of epidermal growth factor by high-performance liquid chromatography

Lynn M. Matrisian, Brent R. Larsen, Joanne S. Finch, Bruce E. Magun

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

Epidermal growth factor (EGF) purified by the method of Savage and Cohen (J. Biol. Chem. 247, 7601-7611 (1972) using DEAE-cellulose chromatography as the final purification step was further resolved into two major uv-absorbing components by reverse-phase high-performance liquid chromatography (HPLC). Both components, referred to as α-EGF and β-EGF, competed with 125I-labeled EGF for the EGF receptor, induced premature eye opening in neonatal mice, and had an amino acid composition similar to that published by Savage et al. (J. Biol. Chem.247, 7612-7621 (1972). β-EGF migrated slightly faster than α-EGF during sodium dodecyl sulfate-urea-polyacrylamide gel electrophoresis. α-EGF was fourfold more potent than β-EGF and was 10-fold more potent than DEAE-purified EGF in stimulating DNA synthesis in quiescent Rat-1 cells. HPLC purification of EGF can replace the DEAE-cellulose chromatography step currently used and produces a more potent and less heterogeneous EGF species.

Original languageEnglish (US)
Pages (from-to)339-351
Number of pages13
JournalAnalytical Biochemistry
Volume125
Issue number2
DOIs
StatePublished - Sep 15 1982

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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