Further evidence against the coupling of dopamine receptors to phosphoinositide hydrolysis in rat striatum

Joan E. Rubinstein, Robert Hitzemann

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The effects of D1 and D2 dopamine receptor agonists on phosphoinositide hydrolysis were studied by measuring the accumulation of radioactive inositol phosphates in slices of rat corpus striatum prelabelled with [3H]inositol. All assays were performed in the presence of lithium. Neither the D1 receptor agonist SKF 38393 nor the D2 receptor agonist quinpirole, alone or in combination, had an effect on basal accumulation of inositol phosphates. The muscarinic receptor agonist carbachol produced a robust increase in the accumulation of inositol monophosphate and a smaller increase in the accumulation of inositol bisphosphate. These effects were not altered by the presence of quinpirole. Additionally, quinpirole also had no effect when assays were conducted in the presence of the muscarinic receptor antagonist scopolamine, the glutamic acid receptor antagonist kynurenic acid, and the antioxidant glutathione. These results are discussed in relation to recent contradictory reports and lend support to the position that D2 dopamine receptors are not coupled to phosphoinositide hydrolysis in rat striatum.

Original languageEnglish (US)
Pages (from-to)1965-1970
Number of pages6
JournalBiochemical Pharmacology
Volume39
Issue number12
DOIs
StatePublished - Jun 15 1990
Externally publishedYes

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Quinpirole
Dopamine Receptors
Inositol
Phosphatidylinositols
Rats
Hydrolysis
Inositol Phosphates
Muscarinic Receptors
Assays
2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine
Kynurenic Acid
Corpus Striatum
Muscarinic Agonists
Muscarinic Antagonists
Scopolamine Hydrobromide
Dopamine D2 Receptors
Dopamine Agonists
Carbachol
Lithium
Glutathione

ASJC Scopus subject areas

  • Pharmacology

Cite this

Further evidence against the coupling of dopamine receptors to phosphoinositide hydrolysis in rat striatum. / Rubinstein, Joan E.; Hitzemann, Robert.

In: Biochemical Pharmacology, Vol. 39, No. 12, 15.06.1990, p. 1965-1970.

Research output: Contribution to journalArticle

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