TY - JOUR
T1 - Functional suppression by FoxP3+CD4+CD25 high regulatory T cells during acute hepatitis C virus infection
AU - Smyk-Pearson, Susan
AU - Golden-Mason, Lucy
AU - Klarquist, Jared
AU - Burton, James R.
AU - Tester, Ian A.
AU - Wang, Chia C.
AU - Culbertson, Nicole
AU - Vandenbark, Arthur A.
AU - Rosen, Hugo R.
N1 - Funding Information:
Received 29 March 2007; accepted 19 June 2007; electronically published 7 December 2007. Potential conflicts of interest: none reported. Presented in part: American Association for the Study of Liver Diseases, San Francisco, 14 November 2005 (abstract 123). Financial support: National Institutes of Health (grant RO1 DK060590 to H.R.R.); HCV Center (grant U19 A 1066328-01 to H.R.R.). a S.S.-P. and L.G.-M. contributed equally to this article. Reprints or correspondence: Dr. Hugo R. Rosen, Waterman Prof. of Medicine and Immunology, Div. of Gastroenterology/Hepatology, UCHSC, GI Division, 4200 E. 9th Ave., B-158, Denver, CO 80262 (regular mail) or 4200 E. 9th Ave., Research Bridge, Rm. 6412, Denver, CO 80220 (express mail) (Hugo.Rosen@UCHSC.edu).
PY - 2008/1/1
Y1 - 2008/1/1
N2 - Background. Infection with hepatitis C virus (HCV) is characterized by impairment of viral effector T cell responses and a high propensity for viral persistence. Previous studies have demonstrated that chronic HCV infection is associated with an increased frequency of regulatory T (Treg) cells, compared with that in persons whose infection resolved and in healthy persons. However, all patients in prior analyses had exposures in the distant past, precluding the ability to determine whether Treg cells play a causal role in establishing persistence during the earliest stages of infection or whether they are expanded because of viral persistence. Methods. For the first time, we longitudinally analyzed Treg cells in patients with acute HCV infection (n = 27). We used a multiparameter approach, including fluorescence-activated cell sorting analysis of cell-surface and intracellular antigens, coculture experiments with highly purified CD4+CD25 high regulatory and CD4+CD25- responder cell populations, and multiplex analysis of secreted cytokines. Results. Forkhead transcription factor 3 (FoxP3) expression and Treg cell suppression were greater in patients with acute HCV infection than in healthy control subjects but were not different at the first time point among patients who subsequently developed persistence or resolved HCV infection spontaneously; however, 6 months later, the resolution of disease was associated with a relative loss of functional suppression. Conclusions. Collectively, these data indicate that patients with acute HCV infection who develop chronicity versus spontaneous resolution exhibit temporal changes in Treg cell function. It is possible that repetitive viral antigenic stimulation alters the function of Treg cells over time.
AB - Background. Infection with hepatitis C virus (HCV) is characterized by impairment of viral effector T cell responses and a high propensity for viral persistence. Previous studies have demonstrated that chronic HCV infection is associated with an increased frequency of regulatory T (Treg) cells, compared with that in persons whose infection resolved and in healthy persons. However, all patients in prior analyses had exposures in the distant past, precluding the ability to determine whether Treg cells play a causal role in establishing persistence during the earliest stages of infection or whether they are expanded because of viral persistence. Methods. For the first time, we longitudinally analyzed Treg cells in patients with acute HCV infection (n = 27). We used a multiparameter approach, including fluorescence-activated cell sorting analysis of cell-surface and intracellular antigens, coculture experiments with highly purified CD4+CD25 high regulatory and CD4+CD25- responder cell populations, and multiplex analysis of secreted cytokines. Results. Forkhead transcription factor 3 (FoxP3) expression and Treg cell suppression were greater in patients with acute HCV infection than in healthy control subjects but were not different at the first time point among patients who subsequently developed persistence or resolved HCV infection spontaneously; however, 6 months later, the resolution of disease was associated with a relative loss of functional suppression. Conclusions. Collectively, these data indicate that patients with acute HCV infection who develop chronicity versus spontaneous resolution exhibit temporal changes in Treg cell function. It is possible that repetitive viral antigenic stimulation alters the function of Treg cells over time.
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U2 - 10.1086/523651
DO - 10.1086/523651
M3 - Article
C2 - 18171284
AN - SCOPUS:39349117113
SN - 0022-1899
VL - 197
SP - 46
EP - 57
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 1
ER -