Functional expression of a myo-inositol/H+ symporter from Leishmania donovani

M. E. Drew, C. K. Langford, E. M. Klamo, D. G. Russell, M. P. Kavanaugh, Scott Landfear

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The vast majority of surface molecules in such kinetoplastid protozoa as members of the genus Leishmania contain inositol and are either glycosyl inositol phospholipids or glycoproteins that are tethered to the external surface of the plasma membrane by glycosylphosphatidylinositol anchors. We have shown that the biosynthetic precursor for these abundant glycolipids, myo-inositol, is translocated across the parasite plasma membrane by a specific transporter that is structurally related to mammalian facilitative glucose transporters. This myo-inositol transporter has been expressed and characterized in Xenopus laevis oocytes. Two-electrode voltage clamp experiments demonstrate that this protein is a sodium-independent electrogenic symporter that appears to utilize a proton gradient to concentrate myo-inositol within the cell. Immunolocalization experiments with a transporter-specific polyclonal antibody reveal the presence of this protein in the parasite plasma membrane.

Original languageEnglish (US)
Pages (from-to)5508-5515
Number of pages8
JournalMolecular and Cellular Biology
Volume15
Issue number10
StatePublished - 1995

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Symporters
Leishmania donovani
Inositol
Cell Membrane
Parasites
Glycosylphosphatidylinositols
Facilitative Glucose Transport Proteins
Leishmania
Glycolipids
Xenopus laevis
Phosphatidylinositols
Oocytes
Protons
Glycoproteins
Electrodes
Proteins
Sodium
Antibodies

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Drew, M. E., Langford, C. K., Klamo, E. M., Russell, D. G., Kavanaugh, M. P., & Landfear, S. (1995). Functional expression of a myo-inositol/H+ symporter from Leishmania donovani. Molecular and Cellular Biology, 15(10), 5508-5515.

Functional expression of a myo-inositol/H+ symporter from Leishmania donovani. / Drew, M. E.; Langford, C. K.; Klamo, E. M.; Russell, D. G.; Kavanaugh, M. P.; Landfear, Scott.

In: Molecular and Cellular Biology, Vol. 15, No. 10, 1995, p. 5508-5515.

Research output: Contribution to journalArticle

Drew, ME, Langford, CK, Klamo, EM, Russell, DG, Kavanaugh, MP & Landfear, S 1995, 'Functional expression of a myo-inositol/H+ symporter from Leishmania donovani', Molecular and Cellular Biology, vol. 15, no. 10, pp. 5508-5515.
Drew ME, Langford CK, Klamo EM, Russell DG, Kavanaugh MP, Landfear S. Functional expression of a myo-inositol/H+ symporter from Leishmania donovani. Molecular and Cellular Biology. 1995;15(10):5508-5515.
Drew, M. E. ; Langford, C. K. ; Klamo, E. M. ; Russell, D. G. ; Kavanaugh, M. P. ; Landfear, Scott. / Functional expression of a myo-inositol/H+ symporter from Leishmania donovani. In: Molecular and Cellular Biology. 1995 ; Vol. 15, No. 10. pp. 5508-5515.
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