Function curve of the membranes that regulate amniotic fluid volume in sheep

Job Faber, Debra Anderson, Alan (Roger) Hohimer, Qin Yang, George Giraud, Lowell Davis

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Seven singleton 120-day fetal lambs were prepared with a shunt from the lung to the gastric end of the esophagus, a bladder catheter, and multiple amniotic fluid and vascular catheters. The urachus was ligated. Beginning 7 days later, amniotic fluid volumes were determined by drainage, followed by replacement with 1 liter of lactated Ringer (LR) solution. Urine flow into the amnion was measured continuously. In 14 of 27 experiments, amniotic fluid volumes were determined again 2 days after the inflow into the amnion had consisted of urine only and in 13 experiments after the inflow of urine had been supplemented by an intraamniotic infusion of LR solution. Intramembranous absorption was calculated from the inflows and the changes in volume between the beginning and end of each experiment. The relations between absorption rate and amniotic fluid volume, the "function curves," were highly individual. Urine production during the infusion of LR solution did not decrease, fetal-plasma renin activity decreased (P <0.001), and amniotic fluid volume increased by 140% [SE (27%), P <0.005], but the increase in the amniochorionic absorption rate of 411% [SE (48%), P <0.001] was greater (P <0.005) than the increase in volume. Each of the seven fetuses was proven capable of an average intramembranous absorption rate that exceeded 4.5 liters of amniotic fluid per day. During the infusion of LR solution, the increase in the rate of absorption matched the rate of infusion (both in ml/h), with a regression coefficient of 0.75 (P <0.001). Thus, even for large amniotic fluid volumes, volume is not limited by the absorptive capacity of the amniochorion, and, at least in these preparations, the position of the function curve and not the natural rate of inflow was the major determinant of resting amniotic fluid volume.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume289
Issue number1 58-1
DOIs
StatePublished - Jul 2005

Fingerprint

Amniotic Fluid
Sheep
Membranes
Urine
Amnion
Urachus
Vascular Access Devices
Renin
Esophagus
Drainage
Stomach
Urinary Bladder
Fetus
Catheters
Lung
Ringer's lactate
Ringer's solution

Keywords

  • Amniochorion
  • Fetus
  • Intramembranous absorption
  • Polyhydramnios

ASJC Scopus subject areas

  • Physiology

Cite this

Function curve of the membranes that regulate amniotic fluid volume in sheep. / Faber, Job; Anderson, Debra; Hohimer, Alan (Roger); Yang, Qin; Giraud, George; Davis, Lowell.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 289, No. 1 58-1, 07.2005.

Research output: Contribution to journalArticle

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