Formaldehyde-induced genome instability is suppressed by an XPF-dependent pathway

Anuradha Kumari, Yun Xin Lim, Amy Hanlon Newell, Susan Olson, Amanda McCullough

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Formaldehyde is a reactive chemical that is commonly used in the production of industrial, laboratory, household, and cosmetic products. The causal association between formaldehyde exposure and increased incidence of cancer led the International Agency for Research on Cancer to classify formaldehyde as a carcinogen. Formaldehyde-induced DNA-protein crosslinks (DPCs) elicit responses involving nucleotide excision repair (NER) and homologous recombination (HR) repair pathways; however, little is known about the cellular and genetic changes that subsequently lead to formaldehyde-induced genotoxic and cytotoxic effects. Herein, investigations of genes that modulate the cytotoxic effects of formaldehyde exposure revealed that of five NER-deficient Chinese Hamster Ovary (CHO) cell lines tested, XPF- and ERCC1-deficient cells were most sensitive to formaldehyde treatment as compared to wild-type cells. Cell cycle analyses revealed that formaldehyde-treated XPF-deficient cells exhibited an immediate G2/M arrest that was associated with altered cell ploidy and apoptosis. Additionally, an elevated number of DNA double-strand breaks (DSBs), chromosomal breaks and radial formation were also observed in XPF-deficient cells following formaldehyde treatment. Formaldehyde-induced DSBs occurred in a replication-dependent, but an XPF-independent manner. However, delayed DSB repair was observed in the absence of XPF function. Collectively, our findings highlight the role of an XPF-dependent pathway in mitigating the sensitivity to formaldehyde-induced DNA damage as evidenced by the increased genomic instability and reduced cell viability in an XPF-deficient background. In addition, centrosome and microtubule abnormalities, as well as enlarged nuclei, caused by formaldehyde exposure are demonstrated in a repair-proficient cell line.

Original languageEnglish (US)
Pages (from-to)236-246
Number of pages11
JournalDNA Repair
Volume11
Issue number3
DOIs
StatePublished - Mar 1 2012

Fingerprint

Genomic Instability
Formaldehyde
Genes
Repair
Cells
DNA Repair
Household Products
International Agencies
Recombinational DNA Repair
Chromosome Breakage
Cell Line
DNA
Centrosome
Double-Stranded DNA Breaks
Nucleotides
Ploidies
Industrial laboratories
Cricetulus
Cosmetics
Microtubules

Keywords

  • DNA-protein crosslink
  • DSBs
  • Formaldehyde
  • Nucleotide excision repair
  • XPF

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Formaldehyde-induced genome instability is suppressed by an XPF-dependent pathway. / Kumari, Anuradha; Lim, Yun Xin; Newell, Amy Hanlon; Olson, Susan; McCullough, Amanda.

In: DNA Repair, Vol. 11, No. 3, 01.03.2012, p. 236-246.

Research output: Contribution to journalArticle

Kumari, Anuradha ; Lim, Yun Xin ; Newell, Amy Hanlon ; Olson, Susan ; McCullough, Amanda. / Formaldehyde-induced genome instability is suppressed by an XPF-dependent pathway. In: DNA Repair. 2012 ; Vol. 11, No. 3. pp. 236-246.
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