Fluid shear stress alters the hemostatic properties of endothelial outgrowth cells

Ann E. Ensley, Robert M. Nerem, Deirdre E J Anderson, Stephen R. Hanson, Monica Hinds

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Surface endothelialization is an attractive means to improve the performance of small diameter vascular grafts. While endothelial outgrowth cells (EOCs) are considered a promising source of autologous endothelium, the ability of EOCs to modulate coagulation-related blood activities is not well understood. The goal of this study was to assess the role of arterial flow conditions on the thrombogenic phenotype of EOCs. EOCs derived from baboon peripheral blood, as well as mature arterial endothelial cells from baboons, were seeded onto adsorbed collagen, then exposed to physiologic levels of fluid shear stress. For important hemostatic pathways, cellular responses to shear stress were characterized at the gene and protein level and confirmed with a functional assay for activated protein C (APC) activity. For EOCs, fluid shear stress upregulated gene and protein expression of anticoagulant and platelet inhibitory factors, including thrombomodulin, tissue factor pathway inhibitor, and nitric oxide synthase 3 (eNOS). Fluid shear stress significantly altered the functional activity of EOCs by increasing APC levels. This study demonstrates that fluid shear stress is an important determinant of EOC hemostatic properties. Accordingly, manipulation of EOC phenotype by mechanical forces may be important for the development of thrombo-resistant surfaces on engineered vascular implants.

Original languageEnglish (US)
Pages (from-to)127-136
Number of pages10
JournalTissue Engineering - Part A
Volume18
Issue number1-2
DOIs
StatePublished - Jan 1 2012

Fingerprint

Hemostatics
Shear stress
Endothelial Cells
Fluids
Proteins
Protein C
Blood
Genes
Thrombomodulin
Papio
Endothelial cells
Nitric oxide
Platelets
Coagulation
Blood Vessels
Collagen
Grafts
Nitric Oxide Synthase
Anticoagulants
Assays

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials
  • Medicine(all)

Cite this

Fluid shear stress alters the hemostatic properties of endothelial outgrowth cells. / Ensley, Ann E.; Nerem, Robert M.; Anderson, Deirdre E J; Hanson, Stephen R.; Hinds, Monica.

In: Tissue Engineering - Part A, Vol. 18, No. 1-2, 01.01.2012, p. 127-136.

Research output: Contribution to journalArticle

Ensley, Ann E. ; Nerem, Robert M. ; Anderson, Deirdre E J ; Hanson, Stephen R. ; Hinds, Monica. / Fluid shear stress alters the hemostatic properties of endothelial outgrowth cells. In: Tissue Engineering - Part A. 2012 ; Vol. 18, No. 1-2. pp. 127-136.
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