Suspensions of metaphase chromosomes from chinese hamster, Indian muntjac and human tissue culture cells were stained for DNA with the fluorochrome, ethidium bromide, and analyzed by flow cytometry to produce a DNA distribution or flow karyotype unique for each species. The flow karyotype is a representation of chromosomal DNA content and relative chromosome frequency. The time for the measurement and data reduction is about ten minutes per 106 chromosomes. Aberrant chromosomes have been detected by flow karyotype changes. Improvements of flow karyotyping and the feasibility of this technique for monitoring human populations for cytogenetic alterations are discussed.
|Original language||English (US)|
|Number of pages||20|
|Journal||LAWRENCE LIVERMORE LAB., U.CALIF.|
|State||Published - Jan 1 1976|
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