Flow cytometry of isolated nuclei prepared from gL rat brain tumor

T. Hoshino, Joe Gray, K. Nomura

Research output: Contribution to journalArticle

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Abstract

Nuclei isolated by grinding 9L rat brain tumors gave a DNA distribution consisting of a 2C DNA peak due to noncycling normal cells and a bimodal distribution beginning at about 4C DNA due to the cycling tumor cells. Autoradiographic studies of nuclei from rats that received a pulse of 3H-TdR in vitro confirmed that the majority of proliferating cells were in the bimodal part of the distribution. The fraction of cells in S-phase determined from the DNA distributions was 15.3%. This is consistent with a labeling index of 18% determined from autoradiographs from tumor tissue and an S-phase fraction of 17% determined from a fraction of labeled mitosis analysis (assuming that G(o)). of the 53% noncycling cells are in G0). The authors also determined by audoradiographic analysis of cells sorted from the tumor's mid-S-phase region, that only 58% of these cells were labeled. They attribute this mostly to the presence of fluorescent debris from necrotic nuclei in this part of the distribution.

Original languageEnglish (US)
Pages (from-to)72-76
Number of pages5
JournalLaboratory Investigation
Volume41
Issue number1
StatePublished - 1979
Externally publishedYes

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Brain Neoplasms
Flow Cytometry
S Phase
DNA
Neoplasms
Mitosis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Flow cytometry of isolated nuclei prepared from gL rat brain tumor. / Hoshino, T.; Gray, Joe; Nomura, K.

In: Laboratory Investigation, Vol. 41, No. 1, 1979, p. 72-76.

Research output: Contribution to journalArticle

Hoshino, T. ; Gray, Joe ; Nomura, K. / Flow cytometry of isolated nuclei prepared from gL rat brain tumor. In: Laboratory Investigation. 1979 ; Vol. 41, No. 1. pp. 72-76.
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