Flow cytometric characterization of a Chinese hamster x man hybrid cell line retaining the human Y chromosome

C. Cremer, Joe Gray, H. H. Ropers

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Abstract

A Chinese hamster x man hybrid cell line (CH-Y-VII) was established which retains a free human Y chromosome. Exponentially growing CH-Y-VII cells were arrested with colcemid; metaphase chromosomes were isolated and stained with 33258 Hoechst (HO) plus Chromomycin A3 (CA3), or with ethidium bromide (EB). The HO/CA3-stained chromosomes were measured in a dual beam flow cytometer, and bivariate HO/CA3 flow karyotypes and univariate HO and CA3 flow karyotypes were established. EB-stained chromosomes were analyzed in a modified Becton Dickinson FACS-Sorter. For all three stains used, the human Y chromosome forms a separate peak in univariate flow karyotypes; the optimum resolution was obtained for the HO distribution. In the bivariate HO/CA3 flow karyotype, the peak for the human Y chromosome is completely separated from the Chinese hamster chromosomes.

Original languageEnglish (US)
Pages (from-to)262-266
Number of pages5
JournalHuman Genetics
Volume60
Issue number3
DOIs
Publication statusPublished - May 1982
Externally publishedYes

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ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

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