FISH probes for mouse chromosome identification

Yu Ping Shi, Gayatry Mohapatra, Joyce Miller, Douglas Hanahan, Eric Lander, Paul Gold, Dan Pinkel, Joe Gray

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

P1 clones near the telomeres and centromeres of each mouse chromosome except Y have been selected from a mouse genomic library and mapped using fluorescence in situ hybridization (FISH). Each clone was selected to contain a genetically mapped polymorphic DNA sequence as close as possible to the centromere or telomere of a chromosome. The genetic distance from the various PI clones to the most distal genetically mapped polymorphic sequence ranged from 0 for about half of the clones to 6.7 cM for the probe at the telomere of chromosome 14. The average distance to the most distal or proximal chromosome marker was 1.5 cM. The use of FISH with these probes for mouse chromosome identification during comparative genomic hybridization is illustrated.

Original languageEnglish (US)
Pages (from-to)42-47
Number of pages6
JournalGenomics
Volume45
Issue number1
DOIs
StatePublished - Oct 1 1997

ASJC Scopus subject areas

  • Genetics

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