FISH probes for mouse chromosome identification

Yu Ping Shi, Gayatry Mohapatra, Joyce Miller, Douglas Hanahan, Eric Lander, Paul Gold, Dan Pinkel, Joe Gray

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

P1 clones near the telomeres and centromeres of each mouse chromosome except Y have been selected from a mouse genomic library and mapped using fluorescence in situ hybridization (FISH). Each clone was selected to contain a genetically mapped polymorphic DNA sequence as close as possible to the centromere or telomere of a chromosome. The genetic distance from the various PI clones to the most distal genetically mapped polymorphic sequence ranged from 0 for about half of the clones to 6.7 cM for the probe at the telomere of chromosome 14. The average distance to the most distal or proximal chromosome marker was 1.5 cM. The use of FISH with these probes for mouse chromosome identification during comparative genomic hybridization is illustrated.

Original languageEnglish (US)
Pages (from-to)42-47
Number of pages6
JournalGenomics
Volume45
Issue number1
DOIs
StatePublished - Oct 1 1997
Externally publishedYes

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Fluorescence In Situ Hybridization
Telomere
Clone Cells
Chromosomes
Centromere
Chromosomes, Human, Pair 14
Comparative Genomic Hybridization
Genomic Library
Y Chromosome
Genetic Markers

ASJC Scopus subject areas

  • Genetics

Cite this

Shi, Y. P., Mohapatra, G., Miller, J., Hanahan, D., Lander, E., Gold, P., ... Gray, J. (1997). FISH probes for mouse chromosome identification. Genomics, 45(1), 42-47. https://doi.org/10.1006/geno.1997.4904

FISH probes for mouse chromosome identification. / Shi, Yu Ping; Mohapatra, Gayatry; Miller, Joyce; Hanahan, Douglas; Lander, Eric; Gold, Paul; Pinkel, Dan; Gray, Joe.

In: Genomics, Vol. 45, No. 1, 01.10.1997, p. 42-47.

Research output: Contribution to journalArticle

Shi, YP, Mohapatra, G, Miller, J, Hanahan, D, Lander, E, Gold, P, Pinkel, D & Gray, J 1997, 'FISH probes for mouse chromosome identification', Genomics, vol. 45, no. 1, pp. 42-47. https://doi.org/10.1006/geno.1997.4904
Shi YP, Mohapatra G, Miller J, Hanahan D, Lander E, Gold P et al. FISH probes for mouse chromosome identification. Genomics. 1997 Oct 1;45(1):42-47. https://doi.org/10.1006/geno.1997.4904
Shi, Yu Ping ; Mohapatra, Gayatry ; Miller, Joyce ; Hanahan, Douglas ; Lander, Eric ; Gold, Paul ; Pinkel, Dan ; Gray, Joe. / FISH probes for mouse chromosome identification. In: Genomics. 1997 ; Vol. 45, No. 1. pp. 42-47.
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