FISH probes for mouse chromosome identification

Yu Ping Shi; Gayatry Mohapatra; Joyce Miller; Douglas Hanahan; Eric Lander; Paul Gold; Dan Pinkel; Joe Gray

doi:10.1006/geno.1997.4904

FISH probes for mouse chromosome identification

Yu Ping Shi, Gayatry Mohapatra, Joyce Miller, Douglas Hanahan, Eric Lander, Paul Gold, Dan Pinkel, Joe Gray

Research output: Contribution to journal › Article › peer-review

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Abstract

P1 clones near the telomeres and centromeres of each mouse chromosome except Y have been selected from a mouse genomic library and mapped using fluorescence in situ hybridization (FISH). Each clone was selected to contain a genetically mapped polymorphic DNA sequence as close as possible to the centromere or telomere of a chromosome. The genetic distance from the various PI clones to the most distal genetically mapped polymorphic sequence ranged from 0 for about half of the clones to 6.7 cM for the probe at the telomere of chromosome 14. The average distance to the most distal or proximal chromosome marker was 1.5 cM. The use of FISH with these probes for mouse chromosome identification during comparative genomic hybridization is illustrated.

Original language	English (US)
Pages (from-to)	42-47
Number of pages	6
Journal	Genomics
Volume	45
Issue number	1
DOIs	https://doi.org/10.1006/geno.1997.4904
State	Published - Oct 1 1997
Externally published	Yes

ASJC Scopus subject areas

Genetics

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10.1006/geno.1997.4904

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title = "FISH probes for mouse chromosome identification",

abstract = "P1 clones near the telomeres and centromeres of each mouse chromosome except Y have been selected from a mouse genomic library and mapped using fluorescence in situ hybridization (FISH). Each clone was selected to contain a genetically mapped polymorphic DNA sequence as close as possible to the centromere or telomere of a chromosome. The genetic distance from the various PI clones to the most distal genetically mapped polymorphic sequence ranged from 0 for about half of the clones to 6.7 cM for the probe at the telomere of chromosome 14. The average distance to the most distal or proximal chromosome marker was 1.5 cM. The use of FISH with these probes for mouse chromosome identification during comparative genomic hybridization is illustrated.",

author = "Shi, {Yu Ping} and Gayatry Mohapatra and Joyce Miller and Douglas Hanahan and Eric Lander and Paul Gold and Dan Pinkel and Joe Gray",

note = "Funding Information: This work was performed with support from Genome Systems, Inc. (P.G.); the Of{\textregistered}ce of Health and Environmental Research, U.S. Department of Energy, contract DE-AC-03-76SF00098; USPHS Grants CA45919 (J.G., D.P.), and Vysis (J.G., D.P.).",

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doi = "10.1006/geno.1997.4904",

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T1 - FISH probes for mouse chromosome identification

AU - Shi, Yu Ping

AU - Mohapatra, Gayatry

AU - Miller, Joyce

AU - Hanahan, Douglas

AU - Lander, Eric

AU - Gold, Paul

AU - Pinkel, Dan

AU - Gray, Joe

N1 - Funding Information: This work was performed with support from Genome Systems, Inc. (P.G.); the Of®ce of Health and Environmental Research, U.S. Department of Energy, contract DE-AC-03-76SF00098; USPHS Grants CA45919 (J.G., D.P.), and Vysis (J.G., D.P.).

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N2 - P1 clones near the telomeres and centromeres of each mouse chromosome except Y have been selected from a mouse genomic library and mapped using fluorescence in situ hybridization (FISH). Each clone was selected to contain a genetically mapped polymorphic DNA sequence as close as possible to the centromere or telomere of a chromosome. The genetic distance from the various PI clones to the most distal genetically mapped polymorphic sequence ranged from 0 for about half of the clones to 6.7 cM for the probe at the telomere of chromosome 14. The average distance to the most distal or proximal chromosome marker was 1.5 cM. The use of FISH with these probes for mouse chromosome identification during comparative genomic hybridization is illustrated.

AB - P1 clones near the telomeres and centromeres of each mouse chromosome except Y have been selected from a mouse genomic library and mapped using fluorescence in situ hybridization (FISH). Each clone was selected to contain a genetically mapped polymorphic DNA sequence as close as possible to the centromere or telomere of a chromosome. The genetic distance from the various PI clones to the most distal genetically mapped polymorphic sequence ranged from 0 for about half of the clones to 6.7 cM for the probe at the telomere of chromosome 14. The average distance to the most distal or proximal chromosome marker was 1.5 cM. The use of FISH with these probes for mouse chromosome identification during comparative genomic hybridization is illustrated.

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FISH probes for mouse chromosome identification

Abstract

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