Fiber delivered probe for efficient CARS imaging of tissues

Mihaela Balu, Gangjun Liu, Zhongping Chen, Bruce J. Tromberg, Eric O. Potma

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

We demonstrate a fiber-based probe for maximum collection of the coherent anti-Stokes Raman scattering (CARS) signal in biological tissues. We discuss the design challenges including capturing the backscattered forward generated CARS signal in the sample and the effects of fiber nonlinearities on the propagating pulses. Three different single mode fibers (fused silica fiber, photonic crystal fiber and double-clad photonic crystal fiber) were tested for the probe design. We investigated self-phase modulation, stimulated Raman scattering (SRS) and four-wave-mixing (FWM) generation in the fiber: nonlinear processes expected to occur in a two-beam excitation based probe. While SPM and SRS induced spectral broadening was negligible, a strong non phase-matched FWM contribution was found to be present in all the tested fibers for excitation conditions relevant to CARS microscopy of tissues. To spectrally suppress this strong contribution, the probe design incorporates separate fibers for excitation light delivery and for signal detection, in combination with dichroic optics. CARS images of the samples were recorded by collecting the back-scattered forward generated CARS signal in the sample through a multi-mode fiber. Different biological tissues were imaged ex vivo in order to assess the performance of our fiber-delivered probe for CARS imaging, a tool which we consider an important advance towards label-free, in vivo probing of superficial tissues.

Original languageEnglish (US)
Pages (from-to)2380-2388
Number of pages9
JournalOptics Express
Volume18
Issue number3
DOIs
StatePublished - Feb 1 2010
Externally publishedYes

Fingerprint

Raman spectra
fibers
probes
four-wave mixing
photonics
excitation
signal detection
phase modulation
crystals
delivery
nonlinearity
optics
silicon dioxide
microscopy
pulses

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Balu, M., Liu, G., Chen, Z., Tromberg, B. J., & Potma, E. O. (2010). Fiber delivered probe for efficient CARS imaging of tissues. Optics Express, 18(3), 2380-2388. https://doi.org/10.1364/OE.18.002380

Fiber delivered probe for efficient CARS imaging of tissues. / Balu, Mihaela; Liu, Gangjun; Chen, Zhongping; Tromberg, Bruce J.; Potma, Eric O.

In: Optics Express, Vol. 18, No. 3, 01.02.2010, p. 2380-2388.

Research output: Contribution to journalArticle

Balu, M, Liu, G, Chen, Z, Tromberg, BJ & Potma, EO 2010, 'Fiber delivered probe for efficient CARS imaging of tissues', Optics Express, vol. 18, no. 3, pp. 2380-2388. https://doi.org/10.1364/OE.18.002380
Balu M, Liu G, Chen Z, Tromberg BJ, Potma EO. Fiber delivered probe for efficient CARS imaging of tissues. Optics Express. 2010 Feb 1;18(3):2380-2388. https://doi.org/10.1364/OE.18.002380
Balu, Mihaela ; Liu, Gangjun ; Chen, Zhongping ; Tromberg, Bruce J. ; Potma, Eric O. / Fiber delivered probe for efficient CARS imaging of tissues. In: Optics Express. 2010 ; Vol. 18, No. 3. pp. 2380-2388.
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