Fast calcium transients in rat peritoneal mast cells are not sufficient to trigger exocytosis.

E. Neher, Wolfhard Almers

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The calcium concentration, [Ca]i, in single rat peritoneal mast cells was measured by means of the new Ca indicator dye fura-2. Upon stimulation with antigen or compound 48/80, [Ca]i rose for seconds to values greater than 5 microM. These Ca transients did not depend on the presence of extracellular Ca, and they sometimes occurred spontaneously, especially in the presence of exogenous phosphatidylserine. Calcium transients did not necessarily lead to degranulation. Degranulation usually occurred during periods of somewhat elevated [Ca] (0.5-1 microM) following transients but was sometimes observed at [Ca]i less than or equal to 250 nM. We found no evidence that an antigen-induced Ca influx is required for degranulation.

Original languageEnglish (US)
Pages (from-to)51-53
Number of pages3
JournalThe EMBO journal
Volume5
Issue number1
StatePublished - Jan 1986
Externally publishedYes

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Exocytosis
Mast Cells
Rats
Calcium
p-Methoxy-N-methylphenethylamine
Antigens
Fura-2
Phosphatidylserines
Coloring Agents
2-(2-(2-chloro-3-(2-(3,3-dimethyl-5-sulfo-1-(4-sulfo-butyl)-3H-indol-2-yl)-vinyl)-cyclohex-2-enylidene)-ethylidene)-3,3-dimethyl-1-(4-sulfo-butyl)-2,3-dihydro-1H-indole-5-carboxylic acid

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Fast calcium transients in rat peritoneal mast cells are not sufficient to trigger exocytosis. / Neher, E.; Almers, Wolfhard.

In: The EMBO journal, Vol. 5, No. 1, 01.1986, p. 51-53.

Research output: Contribution to journalArticle

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