Fast calcium transients in rat peritoneal mast cells are not sufficient to trigger exocytosis.

E. Neher, W. Almers

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

The calcium concentration, [Ca]i, in single rat peritoneal mast cells was measured by means of the new Ca indicator dye fura-2. Upon stimulation with antigen or compound 48/80, [Ca]i rose for seconds to values greater than 5 microM. These Ca transients did not depend on the presence of extracellular Ca, and they sometimes occurred spontaneously, especially in the presence of exogenous phosphatidylserine. Calcium transients did not necessarily lead to degranulation. Degranulation usually occurred during periods of somewhat elevated [Ca] (0.5-1 microM) following transients but was sometimes observed at [Ca]i less than or equal to 250 nM. We found no evidence that an antigen-induced Ca influx is required for degranulation.

Original languageEnglish (US)
Pages (from-to)51-53
Number of pages3
JournalThe EMBO journal
Volume5
Issue number1
StatePublished - Jan 1 1986

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint Dive into the research topics of 'Fast calcium transients in rat peritoneal mast cells are not sufficient to trigger exocytosis.'. Together they form a unique fingerprint.

  • Cite this