Failure of monoaminergic and cholinergic receptor blockers to prevent prostaglandin E2 induced luteinizing hormone release

P. G. Harms, Sergio Ojeda, S. M. McCann

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Receptor blocking drugs were used to determine whether adrenergic, dopaminergic, serotoninergic, or cholinergic synapses are involved in mediating the LH release induced by intraventricularly injected PGE2. Prostaglandin E2 (5 μg) was injected into the 3rd ventricle (3rd V) of ovariectomized rats, and plasma LH concentrations before and after treatment were determined by radioimmunoassay. Phentolamine, 20 or 30 μg, or pronethalol, 20 μg (α and β adrenergic receptor blockers, respectively) injected into the 3rd V failed to alter the elevation of plasma LH evoked by PGE2 injected into the ventricle 10 min later. Likewise, LH release following PGE2 was not changed when a dopaminergic blocker, pimozide (0.63 mg/kg, sc), was injected 2 h prior to PGE2. Two antagonists of serotonin, methysergide maleate (3 mg/kg, ip) or cinanserin HCl (1 mg/kg, iv) given 2 h or 45 min before PGE2, respectively, failed to alter the action of PGE2. Atropine (100 or 250 μg) injected into the 3rd V 10 min prior to PGE2 was also ineffective in blocking the increase in plasma LH following PGE2. The results of this study indicate that the effect of PGE2 on LH release is not mediated by adrenergic, dopaminergic, serotoninergic, or cholinergic receptors. They also suggest that PGE2 is not acting trans synaptically but probably directly on the LHRH neuron to induce the discharge of LHRH into the hypophysial portal vessels which then evokes release of LH from the adenohypophysis.

Original languageEnglish (US)
Pages (from-to)318-323
Number of pages6
JournalEndocrinology
Volume98
Issue number2
StatePublished - 1976
Externally publishedYes

Fingerprint

Cholinergic Receptors
Luteinizing Hormone
Dinoprostone
Third Ventricle
Gonadotropin-Releasing Hormone
Adrenergic Agents
Cinanserin
Pimozide
Drug Receptors
Methysergide
Serotonin Antagonists
Anterior Pituitary Gland
Adrenergic Antagonists
Phentolamine
Atropine
Synapses
Adrenergic Receptors
Cholinergic Agents
Radioimmunoassay
Neurons

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Failure of monoaminergic and cholinergic receptor blockers to prevent prostaglandin E2 induced luteinizing hormone release. / Harms, P. G.; Ojeda, Sergio; McCann, S. M.

In: Endocrinology, Vol. 98, No. 2, 1976, p. 318-323.

Research output: Contribution to journalArticle

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