Factor xi-deficient mice display reduced inflammation, coagulopathy, and bacterial growth during listeriosis

Deyan Luo, Frank M. Szaba, Lawrence W. Kummer, Lawrence L. Johnson, Erik Tucker, Andras Gruber, David Gailani, Stephen T. Smiley

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In mice infected sublethally with Listeria monocytogenes, fibrin is deposited at low levels within hepatic tissue, where it functions protectively by limiting bacterial growth and suppressing hemorrhagic pathology. Here we demonstrate that mice infected with lethal doses of L. monocytogenes produce higher levels of fibrin and display evidence of systemic coagulopathy (i.e., thrombocytopenia, fibrinogen depletion, and elevated levels of thrombin-antithrombin complexes). When the hepatic bacterial burden exceeds 1×10 6 CFU, levels of hepatic fibrin correlate with the bacterial burden, which also correlates with levels of hepatic mRNA encoding the hemostatic enzyme factor XI (FXI). Gene-targeted FXI-deficient mice show significantly improved survival upon challenge with high doses of L. monocytogenes and also display reduced levels of hepatic fibrin, decreased evidence of coagulopathy, and diminished cytokine production (interleukin-6 [IL-6] and IL-10). While fibrin limits the bacterial burden during sublethal listeriosis in wild-type mice, FXI-deficient mice display a significantly improved capacity to restrain the bacterial burden during lethal listeriosis despite their reduced fibrin levels. They also show less evidence of hepatic necrosis. In conjunction with suboptimal antibiotic therapy, FXI-specific monoclonal antibody 14E11 improves survival when administered therapeutically to wild-type mice challenged with high doses of L. monocytogenes. Together, these findings demonstrate the utility of murine listeriosis as a model for dissecting qualitative differences between protective and pathological host responses and reveal novel roles for FXI in exacerbating inflammation and pathogen burden during a lethal bacterial infection.

Original languageEnglish (US)
Pages (from-to)91-99
Number of pages9
JournalInfection and Immunity
Volume80
Issue number1
DOIs
StatePublished - Jan 2012

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Listeriosis
Factor XI
Fibrin
Listeria monocytogenes
Inflammation
Liver
Growth
Hemostatics
Bacterial Infections
Thrombocytopenia
Interleukin-10
Fibrinogen
Interleukin-6
Necrosis
Monoclonal Antibodies
Pathology
Cytokines
Anti-Bacterial Agents
Messenger RNA
Enzymes

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Factor xi-deficient mice display reduced inflammation, coagulopathy, and bacterial growth during listeriosis. / Luo, Deyan; Szaba, Frank M.; Kummer, Lawrence W.; Johnson, Lawrence L.; Tucker, Erik; Gruber, Andras; Gailani, David; Smiley, Stephen T.

In: Infection and Immunity, Vol. 80, No. 1, 01.2012, p. 91-99.

Research output: Contribution to journalArticle

Luo, Deyan ; Szaba, Frank M. ; Kummer, Lawrence W. ; Johnson, Lawrence L. ; Tucker, Erik ; Gruber, Andras ; Gailani, David ; Smiley, Stephen T. / Factor xi-deficient mice display reduced inflammation, coagulopathy, and bacterial growth during listeriosis. In: Infection and Immunity. 2012 ; Vol. 80, No. 1. pp. 91-99.
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