Extraction protocol and mass spectrometry method for quantification of doxorubicin released locally from prodrugs in tumor tissue

Stuart Ibsen, Yongxuan Su, John Norton, Eran Zahavy, Tomoko Hayashi, Stephen Adams, Wolf Wrasidlo, Sadik Esener

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The localized conversion of inactive doxorubicin prodrug chemotherapeutics to pharmacalogically active forms is difficult to quantify in mouse tumor models because it occurs only in small regions of tissue. The tumor tissue extraction protocol and LC-MS/MS analysis method described here were optimized to obtain a detection limit of 7.8 pg for the activated doxorubicin and 0.36 ng for the doxorubicin prodrug. This method can be useful for determining the biodistribution and activation efficiency for many different doxorubicin prodrugs. It can also be used for quantification of doxorubicin from tumor models that have poor vascularization resulting in low tissue accumulation.

Original languageEnglish (US)
Pages (from-to)768-773
Number of pages6
JournalJournal of Mass Spectrometry
Volume48
Issue number7
DOIs
StatePublished - Jul 1 2013
Externally publishedYes

Keywords

  • LC-MS/MS
  • doxorubicin
  • prodrug
  • tissue extraction

ASJC Scopus subject areas

  • Spectroscopy

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