Extracellular acetylcholine is increased in the nucleus accumbens following the presentation of an aversively conditioned taste stimulus

Gregory Mark, Jason B. Weinberg, Pedro V. Rada, Bartley G. Hoebel

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

To determine if acetylcholine (ACh) is released in the nucleus accumbens in response to a conditioned stimulus (CS) that reminds the animal of an aversive event, in vivo microdialysis was used to monitor extracellular ACh during conditioned taste aversion. Saccharin flavored water (2.5 mM saccharin) was paired twice with nausea induced by i.p. lithium chloride (100 mg/kg). This is normally sufficient to create an aversion to the taste of saccharin, but instead of a preference test, the saccharin solution was squirted directly into the rat's mouth via a cheek catheter during nucleus accumbens microdialysis. The result was a 40% increase in extracellular ACh. We reported earlier that dopamine changes in the opposite direction; it decreases. This suggests that high synaptic ACh and low DA are correlated with an aversive state and cessation of behavior.

Original languageEnglish (US)
Pages (from-to)184-188
Number of pages5
JournalBrain Research
Volume688
Issue number1-2
DOIs
StatePublished - Aug 7 1995
Externally publishedYes

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Saccharin
Nucleus Accumbens
Acetylcholine
Microdialysis
Lithium Chloride
Cheek
Nausea
Mouth
Dopamine
Catheters
Water

Keywords

  • CTA
  • Dopamine
  • Microdialysis

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Extracellular acetylcholine is increased in the nucleus accumbens following the presentation of an aversively conditioned taste stimulus. / Mark, Gregory; Weinberg, Jason B.; Rada, Pedro V.; Hoebel, Bartley G.

In: Brain Research, Vol. 688, No. 1-2, 07.08.1995, p. 184-188.

Research output: Contribution to journalArticle

Mark, Gregory ; Weinberg, Jason B. ; Rada, Pedro V. ; Hoebel, Bartley G. / Extracellular acetylcholine is increased in the nucleus accumbens following the presentation of an aversively conditioned taste stimulus. In: Brain Research. 1995 ; Vol. 688, No. 1-2. pp. 184-188.
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