Expression of the kanamycin resistance gene in a kanamycin-producing strain of Streptomyces kanamyceticus

Michiko Nakano, I. Butsuya, H. Ogawara

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The previously cloned kanamycin resistance gene (kmr) from Streptomyces kanamyceticus ISP5500 was shown to modify the 30S ribosomal subunit in a subunit exchange experiment. The kmr gene, which was normally repressed in S. kanamyceticus, appeared to be induced under growth conditions which activated kanamycin biosynthesis. S1 mapping analysis revealed that the expression of the kmr gene was regulated at the transcriptional level. Acetylation of kanamycin is another resistance mechanism in the kanamycin producer. However, unlike kmr-mediated resistance, the enzyme which catalyzed acetylation was not regulated coordinately with kanamycin biosynthesis.

Original languageEnglish (US)
Pages (from-to)423-430
Number of pages8
JournalJournal of Antibiotics
Volume42
Issue number3
StatePublished - 1989
Externally publishedYes

Fingerprint

Kanamycin Resistance
Kanamycin
Streptomyces
Genes
Acetylation
Ribosome Subunits
Enzymes
Growth

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Expression of the kanamycin resistance gene in a kanamycin-producing strain of Streptomyces kanamyceticus. / Nakano, Michiko; Butsuya, I.; Ogawara, H.

In: Journal of Antibiotics, Vol. 42, No. 3, 1989, p. 423-430.

Research output: Contribution to journalArticle

@article{68123e42aeca472ba7bef58ab23067f9,
title = "Expression of the kanamycin resistance gene in a kanamycin-producing strain of Streptomyces kanamyceticus",
abstract = "The previously cloned kanamycin resistance gene (kmr) from Streptomyces kanamyceticus ISP5500 was shown to modify the 30S ribosomal subunit in a subunit exchange experiment. The kmr gene, which was normally repressed in S. kanamyceticus, appeared to be induced under growth conditions which activated kanamycin biosynthesis. S1 mapping analysis revealed that the expression of the kmr gene was regulated at the transcriptional level. Acetylation of kanamycin is another resistance mechanism in the kanamycin producer. However, unlike kmr-mediated resistance, the enzyme which catalyzed acetylation was not regulated coordinately with kanamycin biosynthesis.",
author = "Michiko Nakano and I. Butsuya and H. Ogawara",
year = "1989",
language = "English (US)",
volume = "42",
pages = "423--430",
journal = "Journal of Antibiotics",
issn = "0021-8820",
publisher = "Japan Antibiotics Research Association",
number = "3",

}

TY - JOUR

T1 - Expression of the kanamycin resistance gene in a kanamycin-producing strain of Streptomyces kanamyceticus

AU - Nakano, Michiko

AU - Butsuya, I.

AU - Ogawara, H.

PY - 1989

Y1 - 1989

N2 - The previously cloned kanamycin resistance gene (kmr) from Streptomyces kanamyceticus ISP5500 was shown to modify the 30S ribosomal subunit in a subunit exchange experiment. The kmr gene, which was normally repressed in S. kanamyceticus, appeared to be induced under growth conditions which activated kanamycin biosynthesis. S1 mapping analysis revealed that the expression of the kmr gene was regulated at the transcriptional level. Acetylation of kanamycin is another resistance mechanism in the kanamycin producer. However, unlike kmr-mediated resistance, the enzyme which catalyzed acetylation was not regulated coordinately with kanamycin biosynthesis.

AB - The previously cloned kanamycin resistance gene (kmr) from Streptomyces kanamyceticus ISP5500 was shown to modify the 30S ribosomal subunit in a subunit exchange experiment. The kmr gene, which was normally repressed in S. kanamyceticus, appeared to be induced under growth conditions which activated kanamycin biosynthesis. S1 mapping analysis revealed that the expression of the kmr gene was regulated at the transcriptional level. Acetylation of kanamycin is another resistance mechanism in the kanamycin producer. However, unlike kmr-mediated resistance, the enzyme which catalyzed acetylation was not regulated coordinately with kanamycin biosynthesis.

UR - http://www.scopus.com/inward/record.url?scp=0024582974&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024582974&partnerID=8YFLogxK

M3 - Article

C2 - 2708136

AN - SCOPUS:0024582974

VL - 42

SP - 423

EP - 430

JO - Journal of Antibiotics

JF - Journal of Antibiotics

SN - 0021-8820

IS - 3

ER -