Expression of proliferating cell nuclear antigen (PCNA)/cyclin during the cell cycle

P. Kurki, M. Vanderlaan, F. Dolbeare, Joe Gray, E. M. Tan

Research output: Contribution to journalArticle

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Abstract

The expression of proliferating cell nuclear antigen (PCNA), also called cyclin, was quantified in the cell lines SP2/0 and MOLT-4 and in mouse splenocytes induced to proliferate in vitro with mitogens. Autoantibody from a patient with systemic lupus erythematosus was used to label PCNA in cell suspensions after the cells had been fixed and permeabilized. In the same cells DNA was stained by propidium iodide. The cells were then analysed by flow cytometry for PCNA and DNA content. The PCNA profiles in proliferating spleen cells and the cell lines were similar. Most G0-G1 cells did not express significant amount of PCNA. A dramatic increase in PCNA immunofluorescence was observed in late G1 cells, and further increases were observed in S-phase cells. G2-M cells showed a reduced level of PCNA immunofluorescence relative to S-phase cells but were still elevated relative to G0-G1 cells. Proliferating cells arrested at the G1-S boundary by exposure to cytosine arabinoside showed an increased PCNA immunofluorescence as compared to unstimulated cells.

Original languageEnglish (US)
Pages (from-to)209-219
Number of pages11
JournalExperimental Cell Research
Volume166
Issue number1
DOIs
StatePublished - 1986
Externally publishedYes

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Cyclins
Proliferating Cell Nuclear Antigen
Cell Cycle
Fluorescent Antibody Technique
S Phase
Cell Line
Propidium
DNA
Cytarabine
Mitogens
Systemic Lupus Erythematosus
Autoantibodies
Suspensions
Flow Cytometry
Spleen

ASJC Scopus subject areas

  • Cell Biology

Cite this

Expression of proliferating cell nuclear antigen (PCNA)/cyclin during the cell cycle. / Kurki, P.; Vanderlaan, M.; Dolbeare, F.; Gray, Joe; Tan, E. M.

In: Experimental Cell Research, Vol. 166, No. 1, 1986, p. 209-219.

Research output: Contribution to journalArticle

Kurki, P. ; Vanderlaan, M. ; Dolbeare, F. ; Gray, Joe ; Tan, E. M. / Expression of proliferating cell nuclear antigen (PCNA)/cyclin during the cell cycle. In: Experimental Cell Research. 1986 ; Vol. 166, No. 1. pp. 209-219.
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