Expression of ovary-specific acidic protein in steroidogenic tissues

A possible role in steroidogenesis

Tadashi Matsumoto, Kazuhiro Minegishi, Hitoshi Ishimoto, Mamoru Tanaka, Jon Hennebold, Takahide Teranishi, Yoshihisa Hattori, Masataka Furuya, Takayuki Higuchi, Satoshi Asai, Hye Kim Seon, Kei Miyakoshi, Yasunori Yoshimura

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    Ovary-specific acidic protein (OSAP) is a novel molecule discovered from a genomic project designed to identify ovary-selective genes in mice. Whereas public databases suggest extraovarian expression of OSAP, its tissue distribution has not yet been well documented. Thus, the expression profile of mouse and human OSAP was determined by quantitative real-time RT-PCR using RNAs isolated from various tissues. The results demonstrate that the human and mouse OSAP expression profiles are similar; OSAP is prominently expressed in steroidogenic tissues with the highest level of expression observed in the adrenal gland. Placenta served as an exception and possessed minimal level of OSAP mRNA. Immunohistochemical studies show that mouse OSAP localizes almost exclusively to the steroid-producing cells of the ovary, adrenal gland, and testis. Consistent with predictions made by several subcellular localization algorithms, dual labeling studies in Y-1 mouse adrenocortical cells indicate OSAP resides in the mitochondria. Because of its abundant expression in steroidogenic cells and mitochondrial localization, a role for OSAP in steroidogenesis was determined. OSAP silencing by specific small interfering RNAs significantly inhibits 8-bromoadenosine-cAMP-induced progesterone production in Y-1 cells. Reduction in OSAP levels results in mitochondrial fragmentation and a decrease in the cellular content of mitochondrial DNA, indicative of decreased mitochondrial abundance. Lastly, 8-bromoadenosine-cAMP does not regulate OSAP protein expression in Y-1 cells as is the case for other steroidogenic components known to be induced by cAMP. Collectively these results suggest that OSAP is involved in steroidogenesis, potentially through its ability to maintain mitochondrial abundance and morphology.

    Original languageEnglish (US)
    Pages (from-to)3353-3359
    Number of pages7
    JournalEndocrinology
    Volume150
    Issue number7
    DOIs
    StatePublished - Jul 2009

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    Ovary
    Proteins
    Adrenal Glands
    Tissue Distribution
    Mitochondrial DNA
    Placenta
    Small Interfering RNA
    Progesterone
    Testis
    Real-Time Polymerase Chain Reaction
    Mitochondria
    Steroids
    Databases
    RNA
    Messenger RNA

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Matsumoto, T., Minegishi, K., Ishimoto, H., Tanaka, M., Hennebold, J., Teranishi, T., ... Yoshimura, Y. (2009). Expression of ovary-specific acidic protein in steroidogenic tissues: A possible role in steroidogenesis. Endocrinology, 150(7), 3353-3359. https://doi.org/10.1210/en.2008-1584

    Expression of ovary-specific acidic protein in steroidogenic tissues : A possible role in steroidogenesis. / Matsumoto, Tadashi; Minegishi, Kazuhiro; Ishimoto, Hitoshi; Tanaka, Mamoru; Hennebold, Jon; Teranishi, Takahide; Hattori, Yoshihisa; Furuya, Masataka; Higuchi, Takayuki; Asai, Satoshi; Seon, Hye Kim; Miyakoshi, Kei; Yoshimura, Yasunori.

    In: Endocrinology, Vol. 150, No. 7, 07.2009, p. 3353-3359.

    Research output: Contribution to journalArticle

    Matsumoto, T, Minegishi, K, Ishimoto, H, Tanaka, M, Hennebold, J, Teranishi, T, Hattori, Y, Furuya, M, Higuchi, T, Asai, S, Seon, HK, Miyakoshi, K & Yoshimura, Y 2009, 'Expression of ovary-specific acidic protein in steroidogenic tissues: A possible role in steroidogenesis', Endocrinology, vol. 150, no. 7, pp. 3353-3359. https://doi.org/10.1210/en.2008-1584
    Matsumoto, Tadashi ; Minegishi, Kazuhiro ; Ishimoto, Hitoshi ; Tanaka, Mamoru ; Hennebold, Jon ; Teranishi, Takahide ; Hattori, Yoshihisa ; Furuya, Masataka ; Higuchi, Takayuki ; Asai, Satoshi ; Seon, Hye Kim ; Miyakoshi, Kei ; Yoshimura, Yasunori. / Expression of ovary-specific acidic protein in steroidogenic tissues : A possible role in steroidogenesis. In: Endocrinology. 2009 ; Vol. 150, No. 7. pp. 3353-3359.
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    AU - Furuya, Masataka

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