Expression of LGR7 in the primate corpus luteum implicates the corpus luteum as a relaxin target organ

Priya B. Maseelall, Aimee Seungdamrong, Gerson Weiss, Andrea S. Wojtczuk, Robert Donnelly, Richard Stouffer, Laura T. Goldsmith

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    7 Citations (Scopus)

    Abstract

    In women, the corpus luteum is the source of circulating relaxin. No previous studies have addressed whether the corpus luteum is also a relaxin target organ. We determined relaxin receptor LGR7 mRNA expression in human term pregnancy corpora lutea and nonhuman primate corpora lutea obtained during the menstrual cycle. Real-time reverse transcription-PCR demonstrated the expression of LGR7 mRNA in both human and rhesus monkey corpora lutea. Rhesus monkey corpora lutea were obtained from naturally cycling animals following documented luteinizing hormone (LH) surges at early, mid-, mid-late, and late luteal phases. Luteal expression of LGR7 mRNA did not show temporal variation. Since the primate corpus luteum is LH dependent, we assessed LGR7 mRNA expression in corpora lutea from rhesus monkeys treated with a gonadotropin-releasing hormone (GnRH) antagonist, which significantly suppressed pituitary LH levels. GnRH antagonist treatment, which also inhibits both progesterone and relaxin production, resulted in a fivefold increase in luteal LGR7 mRNA expression. These data suggest that luteal LGR7 mRNA expression may be regulated by relaxin and/or LH and that the primate corpus luteum is a target organ for relaxin.

    Original languageEnglish (US)
    Title of host publicationAnnals of the New York Academy of Sciences
    Pages147-151
    Number of pages5
    Volume1160
    DOIs
    StatePublished - Apr 2009

    Publication series

    NameAnnals of the New York Academy of Sciences
    Volume1160
    ISSN (Print)00778923
    ISSN (Electronic)17496632

    Fingerprint

    Relaxin
    Corpus Luteum
    Primates
    Luteinizing Hormone
    Messenger RNA
    Hormone Antagonists
    Gonadotropin-Releasing Hormone
    Macaca mulatta
    Transcription
    Progesterone
    Animals
    Pituitary Hormones
    Luteal Phase
    Menstrual Cycle
    Reverse Transcription

    Keywords

    • Corpus luteum
    • LGR7
    • Relaxin
    • Relaxin receptor
    • Rhesus monkey
    • RXFP1

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

    Cite this

    Maseelall, P. B., Seungdamrong, A., Weiss, G., Wojtczuk, A. S., Donnelly, R., Stouffer, R., & Goldsmith, L. T. (2009). Expression of LGR7 in the primate corpus luteum implicates the corpus luteum as a relaxin target organ. In Annals of the New York Academy of Sciences (Vol. 1160, pp. 147-151). (Annals of the New York Academy of Sciences; Vol. 1160). https://doi.org/10.1111/j.1749-6632.2009.03946.x

    Expression of LGR7 in the primate corpus luteum implicates the corpus luteum as a relaxin target organ. / Maseelall, Priya B.; Seungdamrong, Aimee; Weiss, Gerson; Wojtczuk, Andrea S.; Donnelly, Robert; Stouffer, Richard; Goldsmith, Laura T.

    Annals of the New York Academy of Sciences. Vol. 1160 2009. p. 147-151 (Annals of the New York Academy of Sciences; Vol. 1160).

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Maseelall, PB, Seungdamrong, A, Weiss, G, Wojtczuk, AS, Donnelly, R, Stouffer, R & Goldsmith, LT 2009, Expression of LGR7 in the primate corpus luteum implicates the corpus luteum as a relaxin target organ. in Annals of the New York Academy of Sciences. vol. 1160, Annals of the New York Academy of Sciences, vol. 1160, pp. 147-151. https://doi.org/10.1111/j.1749-6632.2009.03946.x
    Maseelall PB, Seungdamrong A, Weiss G, Wojtczuk AS, Donnelly R, Stouffer R et al. Expression of LGR7 in the primate corpus luteum implicates the corpus luteum as a relaxin target organ. In Annals of the New York Academy of Sciences. Vol. 1160. 2009. p. 147-151. (Annals of the New York Academy of Sciences). https://doi.org/10.1111/j.1749-6632.2009.03946.x
    Maseelall, Priya B. ; Seungdamrong, Aimee ; Weiss, Gerson ; Wojtczuk, Andrea S. ; Donnelly, Robert ; Stouffer, Richard ; Goldsmith, Laura T. / Expression of LGR7 in the primate corpus luteum implicates the corpus luteum as a relaxin target organ. Annals of the New York Academy of Sciences. Vol. 1160 2009. pp. 147-151 (Annals of the New York Academy of Sciences).
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