Expression of estrogen receptor (ER)-α and -β immunoreactivity in hippocampal cell cultures with special attention to GABAergic neurons

Jun Da Su, Jian Qiu, Yong Ping Zhong, Xiao Yue Li, Jian Wen Wang, Yi Zhang Chen

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Abstract

This study investigated the expression patterns of estrogen receptor-α (erα) and -β (erβ) in the cultured hippocampal cells of neonatal rats by combined application of cell culture and immunocytochemistry. The results revealed that the expression difference between Erα and Erβ seemed to be not obvious in the cultured hippocampal cells of neonatal rats. Moreover, immunoreactivity for either Erα or Erβ was observed to be localized in the majority of not only neurons but also astrocytes. The coexpression of both Erα and Erβ in the same individual cell was also demonstrated by the double-label immunocytochemistry. Western blot analysis showed that immunoreactivity for Erα in the neonatal hippocampal tissues was much higher than in the adult (became rather weak), although there was not such a great difference of immunoreactivity for Erβ. The data also provide direct evidence for the expression of Er subtypes within GABAergic neurons in hippocampal cell cultures and suggest that estrogen's effect on the hippocampus may be mediated at least in part by its Er-containing Gabaergic neurons.

Original languageEnglish (US)
Pages (from-to)396-402
Number of pages7
JournalJournal of Neuroscience Research
Volume65
Issue number5
DOIs
StatePublished - Sep 1 2001

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GABAergic Neurons
Estrogen Receptors
Cultured Cells
Cell Culture Techniques
Immunohistochemistry
Astrocytes
Hippocampus
Estrogens
Western Blotting
Neurons

Keywords

  • Astrocyte
  • Estrogen receptor
  • Gaba
  • Hippocampus
  • Immunocytochemistry

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Expression of estrogen receptor (ER)-α and -β immunoreactivity in hippocampal cell cultures with special attention to GABAergic neurons. / Su, Jun Da; Qiu, Jian; Zhong, Yong Ping; Li, Xiao Yue; Wang, Jian Wen; Chen, Yi Zhang.

In: Journal of Neuroscience Research, Vol. 65, No. 5, 01.09.2001, p. 396-402.

Research output: Contribution to journalArticle

Su, Jun Da ; Qiu, Jian ; Zhong, Yong Ping ; Li, Xiao Yue ; Wang, Jian Wen ; Chen, Yi Zhang. / Expression of estrogen receptor (ER)-α and -β immunoreactivity in hippocampal cell cultures with special attention to GABAergic neurons. In: Journal of Neuroscience Research. 2001 ; Vol. 65, No. 5. pp. 396-402.
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