Expression of CD45RB and CD27 identifies subsets of CD4+ memory T cells with different capacities to induce B cell differentiation

C. Tortorella, H. Schulze-Koops, R. Thomas, J. B. Splawski, L. S. Davis, L. J. Picker, P. E. Lipsky

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53 Scopus citations

Abstract

The capacity of four subsets of CD4+ memory T cells, defined by expression of CD45RB and CD27, to provide help for B cells was examined. Larger amounts of Ig were induced by CD45RB(dim)CD27- cells compared with the CD45RB(dim)CD27+ population, whereas CD45RB(bright)CD27+ or CD27- cells were poor inducers of Ig synthesis. Mitomycin C treatment, which prevents suppressive activity, markedly enhanced Ig production supported by each subset except for CD45RB(bright)CD27- cells. Mitomycin C treated CD45RB(dim) cells remained the most efficient inducers of Ig production, but no difference was detected between CD27+ and CD27- cells. The subsets also differed in their ability to proliferate and secrete cytokines, but these differences did not explain variations in the capacity to provide help for B cells. Both CD27- subsets exhibited decreased proliferation and uniquely secreted IL-4, with the CD45RB(dim)CD27- subset producing the greatest quantities of IL-4. No differences in IL-2 and IFN-γ, production were found. IL-10 secretion increased with the acquisition of the CD45RB(dim) phenotype and, within the CD45RB(dim) cells, with the loss of CD27. Staining for cytoplasmic cytokines indicated that individual populations of CD27-CD4+ helper T cells produced either IL-4 or IFN-γ, whereas more than half of the IL-4 producers also synthesized IL-2. Finally, the different abilities of CD4+ memory T cell subsets to support B cell differentiation did not relate to variations in the expression of CD40 ligand. These results indicate that within the CD4+ memory T cell population an increase of helper activity associates with the shift from a CD45RB(bright) to a CD45RB(dim) phenotype. Within the CD45RB(dim) subset, the loss of CD27 is associated with a reduction of suppressive activity.

Original languageEnglish (US)
Pages (from-to)149-162
Number of pages14
JournalJournal of Immunology
Volume155
Issue number1
StatePublished - Jan 1 1995

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Tortorella, C., Schulze-Koops, H., Thomas, R., Splawski, J. B., Davis, L. S., Picker, L. J., & Lipsky, P. E. (1995). Expression of CD45RB and CD27 identifies subsets of CD4+ memory T cells with different capacities to induce B cell differentiation. Journal of Immunology, 155(1), 149-162.