Expression and distribution of NADPH oxidase isoforms in human myometrium--role in angiotensin II-induced hypertrophy.

Xiao Lan Cui, Baojun Chang, Leslie Myatt

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The renin-angiotensin system is upregulated in pregnant women and may play a role in myometrial hypertrophy during pregnancy. We examined whether angiotensin II could induce myometrial protein synthesis as determined by (3)H-leucine incorporation in an immortalized human myometrial smooth muscle cell line (ULTR cells). The effects of angiotensin II were mediated by NADPH oxidase because diphenylene iodonium abolished angiotensin II-induced protein synthesis. We investigated gene expression and cellular localization of NADPH oxidase isoforms in ULTR cells and confirmed expression of NOX1, NOX4, and NOX5 in myometrial tissue. Angiotensin II induced a cellular redistribution and upregulation of NOX5 protein without altering NOX1 and NOX4 expression. It seems the effect of angiotensin II relies on the type 1 receptor (AT1), because losartan significantly blocked angiotensin II-induced increase in (3)H-leucine incorporation. We conclude that NADPH oxidase mediates angiotensin II-stimulated protein synthesis downstream of AT1 in myometrium smooth muscle cells.

Original languageEnglish (US)
Pages (from-to)305-312
Number of pages8
JournalBiology of Reproduction
Volume82
Issue number2
DOIs
StatePublished - Feb 2010
Externally publishedYes

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Myometrium
NADPH Oxidase
Angiotensin II
Hypertrophy
Protein Isoforms
Leucine
Smooth Muscle Myocytes
Proteins
Losartan
Renin-Angiotensin System
Pregnant Women
Up-Regulation
Gene Expression
Cell Line
Pregnancy

ASJC Scopus subject areas

  • Cell Biology

Cite this

Expression and distribution of NADPH oxidase isoforms in human myometrium--role in angiotensin II-induced hypertrophy. / Cui, Xiao Lan; Chang, Baojun; Myatt, Leslie.

In: Biology of Reproduction, Vol. 82, No. 2, 02.2010, p. 305-312.

Research output: Contribution to journalArticle

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