Expression and distribution of glucagon-like peptide-1 receptor mrna, protein and binding in the male nonhuman primate (macaca mulatta) brain

Kristy M. Heppner, Melissa Kirigiti, Anna Secher, Sarah Juel Paulsen, Rikley Buckingham, Charles Pyke, Lotte B. Knudsen, Niels Vrang, Kevin Grove

    Research output: Contribution to journalArticle

    51 Citations (Scopus)

    Abstract

    Glucagon-like peptide-1 (GLP-1) is released from endocrine L-cells lining the gut in response to food ingestion. However, GLP-1 is also produced in the nucleus of the solitary tract, where it acts as an anorectic neurotransmitter and key regulator of many autonomic and neuroendocrine functions. The expression and projections of GLP-1-producing neurons is highly conserved between rodent and primate brain, although a few key differences have been identified. The GLP-1 receptor (GLP-1R) has been mapped in the rodent brain, but no studies have described the distribution of GLP-1Rs in the nonhuman primate central nervous system. Here, we characterized the distribution of GLP-1R mRNA and protein in the adult macaque brain using in situ hybridization, radioligand receptor autoradiography, and immunohistochemistry with a primate specific GLP-1R antibody. Immunohistochemistry demonstrated that the GLP-1R is localized to cell bodies and fiber terminals in a very selective distribution throughout the brain. Consistent with the functional role of the GLP-1R system, we find the highest concentration of GLP-1R-immunoreactivity present in select hypothalamic and brainstem regions that regulate feeding, including the paraventricular and arcuate hypothalamic nuclei, as well as the area postrema, nucleus of the solitary tract, and dorsal motor nucleus of the vagus. Together, our data demonstrate that GLP-1R distribution is highly conserved between rodent and primate, although a few key species differences were identified, including the amygdala, where GLP-1R expression is much higher in primate than in rodent.

    Original languageEnglish (US)
    Pages (from-to)255-267
    Number of pages13
    JournalEndocrinology
    Volume156
    Issue number1
    DOIs
    StatePublished - Jan 1 2015

    Fingerprint

    Macaca mulatta
    Protein Binding
    Primates
    Brain
    Glucagon-Like Peptide 1
    Rodentia
    Solitary Nucleus
    Immunohistochemistry
    Area Postrema
    Appetite Depressants
    Arcuate Nucleus of Hypothalamus
    Endocrine Cells
    Glucagon-Like Peptide-1 Receptor
    Paraventricular Hypothalamic Nucleus
    Macaca
    Amygdala
    Autoradiography
    Brain Stem
    In Situ Hybridization
    Neurotransmitter Agents

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Heppner, K. M., Kirigiti, M., Secher, A., Paulsen, S. J., Buckingham, R., Pyke, C., ... Grove, K. (2015). Expression and distribution of glucagon-like peptide-1 receptor mrna, protein and binding in the male nonhuman primate (macaca mulatta) brain. Endocrinology, 156(1), 255-267. https://doi.org/10.1210/en.2014-1675

    Expression and distribution of glucagon-like peptide-1 receptor mrna, protein and binding in the male nonhuman primate (macaca mulatta) brain. / Heppner, Kristy M.; Kirigiti, Melissa; Secher, Anna; Paulsen, Sarah Juel; Buckingham, Rikley; Pyke, Charles; Knudsen, Lotte B.; Vrang, Niels; Grove, Kevin.

    In: Endocrinology, Vol. 156, No. 1, 01.01.2015, p. 255-267.

    Research output: Contribution to journalArticle

    Heppner, KM, Kirigiti, M, Secher, A, Paulsen, SJ, Buckingham, R, Pyke, C, Knudsen, LB, Vrang, N & Grove, K 2015, 'Expression and distribution of glucagon-like peptide-1 receptor mrna, protein and binding in the male nonhuman primate (macaca mulatta) brain', Endocrinology, vol. 156, no. 1, pp. 255-267. https://doi.org/10.1210/en.2014-1675
    Heppner, Kristy M. ; Kirigiti, Melissa ; Secher, Anna ; Paulsen, Sarah Juel ; Buckingham, Rikley ; Pyke, Charles ; Knudsen, Lotte B. ; Vrang, Niels ; Grove, Kevin. / Expression and distribution of glucagon-like peptide-1 receptor mrna, protein and binding in the male nonhuman primate (macaca mulatta) brain. In: Endocrinology. 2015 ; Vol. 156, No. 1. pp. 255-267.
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