Excitatory amino acid receptors in the dorsomedial hypothalamus mediate prostaglandin-evoked thermogenesis in brown adipose tissue

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Abstract

We determined whether the dorsomedial hypothalamus (DMH) plays a role in the thermogenic, metabolic, and cardiovascular effects evoked by centrally administered PGE2. Microinjection of PGE2 (170 pmol/60 nl) into the medial preoptic area of the hypothalamus in urethane-chloralose-anesthetized, artificially ventilated rats increased brown adipose tissue (BAT) sympathetic nerve activity (SNA; + 207 ± 18% of control), BAT temperature (1.5 ± 0.2°C), expired CO2 (0.9 ± 0.1%), heart rate (HR; 106 ± 12 beats/min), and mean arterial pressure (22 ± 4 mmHg). Within 5 min of subsequent bilateral microinjections of the GABAA receptor agonist muscimol (120 pmol·60 nl-1·side-1) or the ionotropic excitatory amino acid antagonist kynurenate (6 nmol·60 nl -1·side-1) into the DMH, the PGE 2-evoked increases were, respectively, attenuated by 91 ± 3% and 108 ± 7% for BAT SNA, by 73 ± 12% and 102 ± 28% for BAT temperature, by 100 ± 4% and 125 ± 21% for expired CO 2, by 72 ± 11% and 70 ± 16% for HR, and by 84 ± 19% and 113 ± 16% for mean arterial pressure. Microinjections outside the DMH within the dorsal hypothalamic area adjacent to the mamillothalamic tracts or within the ventromedial hypothalamus were less effective for attenuating the PGE2-evoked thermogenic, metabolic, and cardiovascular responses. These results demonstrate that activation of excitatory amino acid receptors within the DMH is necessary for the thermogenic, metabolic, and cardiovascular responses evoked by microinjection of PGE 2 into the medial preoptic area.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume286
Issue number2 55-2
StatePublished - Feb 2004

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Brown Adipose Tissue
Thermogenesis
Glutamate Receptors
Hypothalamus
Prostaglandins
Microinjections
Dinoprostone
Preoptic Area
Prostaglandins E
Arterial Pressure
GABA-A Receptor Agonists
Kynurenic Acid
Aminoacylation
Excitatory Amino Acid Antagonists
Muscimol
Chloralose
Temperature
Urethane
Carbon Monoxide
Heart Rate

Keywords

  • Febrile
  • Glutamate
  • Medial preoptic area
  • Sympathetic
  • Thermoregulation

ASJC Scopus subject areas

  • Physiology

Cite this

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title = "Excitatory amino acid receptors in the dorsomedial hypothalamus mediate prostaglandin-evoked thermogenesis in brown adipose tissue",
abstract = "We determined whether the dorsomedial hypothalamus (DMH) plays a role in the thermogenic, metabolic, and cardiovascular effects evoked by centrally administered PGE2. Microinjection of PGE2 (170 pmol/60 nl) into the medial preoptic area of the hypothalamus in urethane-chloralose-anesthetized, artificially ventilated rats increased brown adipose tissue (BAT) sympathetic nerve activity (SNA; + 207 ± 18{\%} of control), BAT temperature (1.5 ± 0.2°C), expired CO2 (0.9 ± 0.1{\%}), heart rate (HR; 106 ± 12 beats/min), and mean arterial pressure (22 ± 4 mmHg). Within 5 min of subsequent bilateral microinjections of the GABAA receptor agonist muscimol (120 pmol·60 nl-1·side-1) or the ionotropic excitatory amino acid antagonist kynurenate (6 nmol·60 nl -1·side-1) into the DMH, the PGE 2-evoked increases were, respectively, attenuated by 91 ± 3{\%} and 108 ± 7{\%} for BAT SNA, by 73 ± 12{\%} and 102 ± 28{\%} for BAT temperature, by 100 ± 4{\%} and 125 ± 21{\%} for expired CO 2, by 72 ± 11{\%} and 70 ± 16{\%} for HR, and by 84 ± 19{\%} and 113 ± 16{\%} for mean arterial pressure. Microinjections outside the DMH within the dorsal hypothalamic area adjacent to the mamillothalamic tracts or within the ventromedial hypothalamus were less effective for attenuating the PGE2-evoked thermogenic, metabolic, and cardiovascular responses. These results demonstrate that activation of excitatory amino acid receptors within the DMH is necessary for the thermogenic, metabolic, and cardiovascular responses evoked by microinjection of PGE 2 into the medial preoptic area.",
keywords = "Febrile, Glutamate, Medial preoptic area, Sympathetic, Thermoregulation",
author = "Madden, {Christopher (Chris)} and Shaun Morrison",
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language = "English (US)",
volume = "286",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
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T1 - Excitatory amino acid receptors in the dorsomedial hypothalamus mediate prostaglandin-evoked thermogenesis in brown adipose tissue

AU - Madden, Christopher (Chris)

AU - Morrison, Shaun

PY - 2004/2

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N2 - We determined whether the dorsomedial hypothalamus (DMH) plays a role in the thermogenic, metabolic, and cardiovascular effects evoked by centrally administered PGE2. Microinjection of PGE2 (170 pmol/60 nl) into the medial preoptic area of the hypothalamus in urethane-chloralose-anesthetized, artificially ventilated rats increased brown adipose tissue (BAT) sympathetic nerve activity (SNA; + 207 ± 18% of control), BAT temperature (1.5 ± 0.2°C), expired CO2 (0.9 ± 0.1%), heart rate (HR; 106 ± 12 beats/min), and mean arterial pressure (22 ± 4 mmHg). Within 5 min of subsequent bilateral microinjections of the GABAA receptor agonist muscimol (120 pmol·60 nl-1·side-1) or the ionotropic excitatory amino acid antagonist kynurenate (6 nmol·60 nl -1·side-1) into the DMH, the PGE 2-evoked increases were, respectively, attenuated by 91 ± 3% and 108 ± 7% for BAT SNA, by 73 ± 12% and 102 ± 28% for BAT temperature, by 100 ± 4% and 125 ± 21% for expired CO 2, by 72 ± 11% and 70 ± 16% for HR, and by 84 ± 19% and 113 ± 16% for mean arterial pressure. Microinjections outside the DMH within the dorsal hypothalamic area adjacent to the mamillothalamic tracts or within the ventromedial hypothalamus were less effective for attenuating the PGE2-evoked thermogenic, metabolic, and cardiovascular responses. These results demonstrate that activation of excitatory amino acid receptors within the DMH is necessary for the thermogenic, metabolic, and cardiovascular responses evoked by microinjection of PGE 2 into the medial preoptic area.

AB - We determined whether the dorsomedial hypothalamus (DMH) plays a role in the thermogenic, metabolic, and cardiovascular effects evoked by centrally administered PGE2. Microinjection of PGE2 (170 pmol/60 nl) into the medial preoptic area of the hypothalamus in urethane-chloralose-anesthetized, artificially ventilated rats increased brown adipose tissue (BAT) sympathetic nerve activity (SNA; + 207 ± 18% of control), BAT temperature (1.5 ± 0.2°C), expired CO2 (0.9 ± 0.1%), heart rate (HR; 106 ± 12 beats/min), and mean arterial pressure (22 ± 4 mmHg). Within 5 min of subsequent bilateral microinjections of the GABAA receptor agonist muscimol (120 pmol·60 nl-1·side-1) or the ionotropic excitatory amino acid antagonist kynurenate (6 nmol·60 nl -1·side-1) into the DMH, the PGE 2-evoked increases were, respectively, attenuated by 91 ± 3% and 108 ± 7% for BAT SNA, by 73 ± 12% and 102 ± 28% for BAT temperature, by 100 ± 4% and 125 ± 21% for expired CO 2, by 72 ± 11% and 70 ± 16% for HR, and by 84 ± 19% and 113 ± 16% for mean arterial pressure. Microinjections outside the DMH within the dorsal hypothalamic area adjacent to the mamillothalamic tracts or within the ventromedial hypothalamus were less effective for attenuating the PGE2-evoked thermogenic, metabolic, and cardiovascular responses. These results demonstrate that activation of excitatory amino acid receptors within the DMH is necessary for the thermogenic, metabolic, and cardiovascular responses evoked by microinjection of PGE 2 into the medial preoptic area.

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KW - Medial preoptic area

KW - Sympathetic

KW - Thermoregulation

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SN - 1931-857X

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