EX-Vivo expansion of neutrophil precursors and megakaryocytes from cd34+ cells using novel hematopoietic factors receptor agonists in serum free media

M. Loudovaris, J. G. Bender, K. Unverzagt, J. Martinson, D. Van Epps, N. I. Minster, Philip Streeter, C. M. Baum, J. P. McKearn

Research output: Contribution to journalArticle

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Abstract

Two major complications of high dose chemotherapy are neutropenia and thrdmbocytopenia. Transfusion of ex vivo expanded blood progenitors could reduce the period of cytopenia by providing mature functional cells within days of infusion. We investigated the ability of threejiovel multi-functional hematopoietic growth factor receptor agonists: Synthokine dimer (Syn), Myelopoietin (MPO) and Promegapoietin (PMP) to generate megakaryocyte (MKs)and neutrophil precursors (NP). CD34+ cells purified using the Isolex(r) 300 from G-CSF mobilized apheresis products were cultured in X-VIVO 10(tm) + 1% Buminate containing Syn, MPO, PMP alone or in combination. Starting CFU content was 16.8E04±2.3. Cultures were evaluated at day 12 for cell numbers, phenotype and colony forming cells. Results are mean+/-SD (n=6). Factors Fold Total CFU % CD15+ %CD41a+ Increase (E04) cells (NP) cells (MK) Syn 77±26 8.1±1.8 46.4±2 4 7.4±3 3 MPO 109+44 109±47 670+13 5 5±1 8 PMP 128±61 154±78 55.3±1 6 9 Ol4 6 Syn+MPO 216±122 149+58 65.1±8.7 4 2±2 1 Syn+PMP 274+13.3 242±96 43.7±7 1 10.3±5.2 MPO+PMP 297±19.5 17.7+30 64.5±6.5 6.4+15 Syn+MPO+PMP 37.0+15.9 30.1+5.2 59.3t5 9 7.0+1.8 All three factors alone or in combination supported CD34+ cell proliferation and differentiation. Addition of Syn to MPO or PMP resulted in a significant increase in the total number of NP and MKs, respectively. MPO/PMP or PMP/MPO/Syn generated the largest number of NP and MKs. Syn/MPO/PMP resulted in a significantly higher number of CFU compared to the other combinations tested (p

Original languageEnglish (US)
Pages (from-to)833
Number of pages1
JournalExperimental Hematology
Volume25
Issue number8
StatePublished - 1997
Externally publishedYes

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Megakaryocytes
Serum-Free Culture Media
Neutrophils
Colony-Stimulating Factor Receptors
myelopoietin
Blood Component Removal
Granulocyte Colony-Stimulating Factor
Neutropenia
Cell Differentiation
Cell Count
Cell Proliferation
Phenotype
Drug Therapy

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Loudovaris, M., Bender, J. G., Unverzagt, K., Martinson, J., Van Epps, D., Minster, N. I., ... McKearn, J. P. (1997). EX-Vivo expansion of neutrophil precursors and megakaryocytes from cd34+ cells using novel hematopoietic factors receptor agonists in serum free media. Experimental Hematology, 25(8), 833.

EX-Vivo expansion of neutrophil precursors and megakaryocytes from cd34+ cells using novel hematopoietic factors receptor agonists in serum free media. / Loudovaris, M.; Bender, J. G.; Unverzagt, K.; Martinson, J.; Van Epps, D.; Minster, N. I.; Streeter, Philip; Baum, C. M.; McKearn, J. P.

In: Experimental Hematology, Vol. 25, No. 8, 1997, p. 833.

Research output: Contribution to journalArticle

Loudovaris, M, Bender, JG, Unverzagt, K, Martinson, J, Van Epps, D, Minster, NI, Streeter, P, Baum, CM & McKearn, JP 1997, 'EX-Vivo expansion of neutrophil precursors and megakaryocytes from cd34+ cells using novel hematopoietic factors receptor agonists in serum free media', Experimental Hematology, vol. 25, no. 8, pp. 833.
Loudovaris, M. ; Bender, J. G. ; Unverzagt, K. ; Martinson, J. ; Van Epps, D. ; Minster, N. I. ; Streeter, Philip ; Baum, C. M. ; McKearn, J. P. / EX-Vivo expansion of neutrophil precursors and megakaryocytes from cd34+ cells using novel hematopoietic factors receptor agonists in serum free media. In: Experimental Hematology. 1997 ; Vol. 25, No. 8. pp. 833.
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abstract = "Two major complications of high dose chemotherapy are neutropenia and thrdmbocytopenia. Transfusion of ex vivo expanded blood progenitors could reduce the period of cytopenia by providing mature functional cells within days of infusion. We investigated the ability of threejiovel multi-functional hematopoietic growth factor receptor agonists: Synthokine dimer (Syn), Myelopoietin (MPO) and Promegapoietin (PMP) to generate megakaryocyte (MKs)and neutrophil precursors (NP). CD34+ cells purified using the Isolex(r) 300 from G-CSF mobilized apheresis products were cultured in X-VIVO 10(tm) + 1{\%} Buminate containing Syn, MPO, PMP alone or in combination. Starting CFU content was 16.8E04±2.3. Cultures were evaluated at day 12 for cell numbers, phenotype and colony forming cells. Results are mean+/-SD (n=6). Factors Fold Total CFU {\%} CD15+ {\%}CD41a+ Increase (E04) cells (NP) cells (MK) Syn 77±26 8.1±1.8 46.4±2 4 7.4±3 3 MPO 109+44 109±47 670+13 5 5±1 8 PMP 128±61 154±78 55.3±1 6 9 Ol4 6 Syn+MPO 216±122 149+58 65.1±8.7 4 2±2 1 Syn+PMP 274+13.3 242±96 43.7±7 1 10.3±5.2 MPO+PMP 297±19.5 17.7+30 64.5±6.5 6.4+15 Syn+MPO+PMP 37.0+15.9 30.1+5.2 59.3t5 9 7.0+1.8 All three factors alone or in combination supported CD34+ cell proliferation and differentiation. Addition of Syn to MPO or PMP resulted in a significant increase in the total number of NP and MKs, respectively. MPO/PMP or PMP/MPO/Syn generated the largest number of NP and MKs. Syn/MPO/PMP resulted in a significantly higher number of CFU compared to the other combinations tested (p",
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T1 - EX-Vivo expansion of neutrophil precursors and megakaryocytes from cd34+ cells using novel hematopoietic factors receptor agonists in serum free media

AU - Loudovaris, M.

AU - Bender, J. G.

AU - Unverzagt, K.

AU - Martinson, J.

AU - Van Epps, D.

AU - Minster, N. I.

AU - Streeter, Philip

AU - Baum, C. M.

AU - McKearn, J. P.

PY - 1997

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