Evolutionary conservation of primate lymphocryptovirus MicroRNA targets

Rebecca Skalsky, Dong Kang, Sarah D. Linnstaedt, Bryan R. Cullen

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Epstein-Barr virus (EBV) and rhesus lymphocryptovirus (rLCV) are closely related gammaherpesviruses in the lymphocryptovirus subgroup that express viral microRNAs (miRNAs) during latent infection. In addition to many host mRNAs, EBV miRNAs are known to target latent viral transcripts, specifically those encoding LMP1, BHRF1, and EBNA2. The mRNA targets of rLCV miRNAs have not been investigated. Using luciferase reporter assays, photoactivatable cross-linking and immunoprecipitation (PAR-CLIP), and deep sequencing, we demonstrate that posttranscriptional regulation of LMP1 expression is a conserved function of lymphocryptovirus miRNAs. Furthermore, the mRNAs encoding the rLCV EBNA2 and BHRF1 homologs are regulated by miRNAs in rLCV-infected B cells. Homologous to sites in the EBV LMP1 and BHRF1 3'-untranslated regions (UTRs), we also identified evolutionarily conserved binding sites for the cellular miR-17/20/106 family in the LMP1 and BHRF1 3'UTRs of several primate LCVs. Finally, we investigated the functional consequences of LMP1 targeting by individual EBV BART miRNAs and show that select viral miRNAs play a role in the previously observed modulation of NF-κB activation.

Original languageEnglish (US)
Pages (from-to)1617-1635
Number of pages19
JournalJournal of Virology
Volume88
Issue number3
DOIs
StatePublished - Feb 2014
Externally publishedYes

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Lymphocryptovirus
Macacine herpesvirus 4
MicroRNAs
microRNA
Primates
Human herpesvirus 4
Human Herpesvirus 4
3' Untranslated Regions
3' untranslated regions
Messenger RNA
High-Throughput Nucleotide Sequencing
luciferase
Luciferases
crosslinking
Immunoprecipitation
B-lymphocytes
binding sites
B-Lymphocytes
Binding Sites

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Evolutionary conservation of primate lymphocryptovirus MicroRNA targets. / Skalsky, Rebecca; Kang, Dong; Linnstaedt, Sarah D.; Cullen, Bryan R.

In: Journal of Virology, Vol. 88, No. 3, 02.2014, p. 1617-1635.

Research output: Contribution to journalArticle

Skalsky, Rebecca ; Kang, Dong ; Linnstaedt, Sarah D. ; Cullen, Bryan R. / Evolutionary conservation of primate lymphocryptovirus MicroRNA targets. In: Journal of Virology. 2014 ; Vol. 88, No. 3. pp. 1617-1635.
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