Evidence that ecotropic murine leukemia virus contamination in TZM-bl cells does not affect the outcome of neutralizing antibody assays with human immunodeficiency virus type 1

Emily J. Platt, Miroslawa Bilska, Susan L. Kozak, David Kabat, David C. Montefiori

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

The TZM-bl cell line that is commonly used to assess neutralizing antibodies against human immunodeficiency virus type 1 (HIV-1) was recently reported to be contaminated with an ecotropic murine leukemia virus (MLV) (Y. Takeuchi, M. O. McClure, and M. Pizzato, J. Virol. 82:12585-12588, 2008), raising questions about the validity of results obtained with this cell line. Here we confirm this observation and show that HIV-1 neutralization assays performed with a variety of serologic reagents in a similar cell line that does not harbor MLV yield results that are equivalent to those obtained in TZM-bl cells. We conclude that MLV contamination has no measurable effect on HIV-1 neutralization when TZM-bl cells are used as targets for infection.

Original languageEnglish (US)
Pages (from-to)8289-8292
Number of pages4
JournalJournal of Virology
Volume83
Issue number16
DOIs
StatePublished - Aug 2009

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Murine leukemia virus
Murine Leukemia Viruses
Human immunodeficiency virus 1
Neutralizing Antibodies
neutralizing antibodies
HIV-1
cell lines
Cell Line
assays
neutralization tests
cells
Reproducibility of Results
neutralization
Infection
infection

ASJC Scopus subject areas

  • Immunology
  • Virology

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Evidence that ecotropic murine leukemia virus contamination in TZM-bl cells does not affect the outcome of neutralizing antibody assays with human immunodeficiency virus type 1. / Platt, Emily J.; Bilska, Miroslawa; Kozak, Susan L.; Kabat, David; Montefiori, David C.

In: Journal of Virology, Vol. 83, No. 16, 08.2009, p. 8289-8292.

Research output: Contribution to journalArticle

Platt, Emily J. ; Bilska, Miroslawa ; Kozak, Susan L. ; Kabat, David ; Montefiori, David C. / Evidence that ecotropic murine leukemia virus contamination in TZM-bl cells does not affect the outcome of neutralizing antibody assays with human immunodeficiency virus type 1. In: Journal of Virology. 2009 ; Vol. 83, No. 16. pp. 8289-8292.
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