Evaluation of a novel alloplast for osseous regeneration in the rat calvarial model

Paul O. Francis, James C. McPherson, Michael F. Cuenin, Steven Hokett, Mark E. Peacock, Michael A. Billman, Mohamed Sharawy

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Alloplasts are inert foreign bodies acting as osteoconductive space maintainers during osseous wound healing. They may also function as carriers for growth factors that are known to enhance neovascularization and osteoinduction; human recombinant transforming growth factor beta (rhTGF-β1) is one such factor. The purpose of this study was to evaluate di-vinyl styrene beads (DVSb) and rhTGF-β1 effects on osseous regeneration in the rat calvaria critical-sized defect model. Methods: Di-vinyl styrene beads (DVSb) with and without rhTGF-β1 were placed between gelfilm membranes in a critical-sized defect in the rat (Rattus norvegicus) calvaria. Actual bone fill; percentage bone fill; bone formation rate (BFR); and mineralization at 3, 6, and 12 weeks postsurgery were measured using densitometry, histomorphometry, scanning electron microscopy (SEM), and vital staining with tetracycline-HCI. Results: Mean radiographic density and percentage fill were statistically greater for DVSb treatment groups as compared with controls (P ≤0.05). BFR was consistently between 3 and 7 μm per day for all groups; alloplast group BFR was significantly greater than controls or the rhTGF-β1 groups at 6 weeks (P ≤0.05); however, at 3 and 12 weeks, the control BFR was greater than treatment groups (P ≤0.05). Membranes often collapsed and little bone fill or mineralization occurred in defects without DVSb. Mineralization appeared to occur adjacent to the alloplast by 12 weeks in the histologic and SEM sections. While DVSb fibrous attachment occurred in some specimens, there was no evidence of an inflammatory response. Conclusion: Di-vinyl styrene beads, with or without rhTGF-β1, significantly enhance bone regeneration in the rat calvaria defect model.

Original languageEnglish (US)
Pages (from-to)1023-1031
Number of pages9
JournalJournal of Periodontology
Volume74
Issue number7
DOIs
StatePublished - Jul 1 2003
Externally publishedYes

Fingerprint

Styrene
Regeneration
Osteogenesis
Skull
Bone and Bones
Electron Scanning Microscopy
Bone Regeneration
Membranes
Densitometry
Foreign Bodies
Tetracycline
Transforming Growth Factor beta
Wound Healing
Intercellular Signaling Peptides and Proteins
Staining and Labeling

Keywords

  • Animal studies
  • Bone regeneration
  • Foreign bodies
  • Growth factors, transforming
  • Models, animal
  • Wound healing

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Francis, P. O., McPherson, J. C., Cuenin, M. F., Hokett, S., Peacock, M. E., Billman, M. A., & Sharawy, M. (2003). Evaluation of a novel alloplast for osseous regeneration in the rat calvarial model. Journal of Periodontology, 74(7), 1023-1031. https://doi.org/10.1902/jop.2003.74.7.1023

Evaluation of a novel alloplast for osseous regeneration in the rat calvarial model. / Francis, Paul O.; McPherson, James C.; Cuenin, Michael F.; Hokett, Steven; Peacock, Mark E.; Billman, Michael A.; Sharawy, Mohamed.

In: Journal of Periodontology, Vol. 74, No. 7, 01.07.2003, p. 1023-1031.

Research output: Contribution to journalArticle

Francis, PO, McPherson, JC, Cuenin, MF, Hokett, S, Peacock, ME, Billman, MA & Sharawy, M 2003, 'Evaluation of a novel alloplast for osseous regeneration in the rat calvarial model', Journal of Periodontology, vol. 74, no. 7, pp. 1023-1031. https://doi.org/10.1902/jop.2003.74.7.1023
Francis, Paul O. ; McPherson, James C. ; Cuenin, Michael F. ; Hokett, Steven ; Peacock, Mark E. ; Billman, Michael A. ; Sharawy, Mohamed. / Evaluation of a novel alloplast for osseous regeneration in the rat calvarial model. In: Journal of Periodontology. 2003 ; Vol. 74, No. 7. pp. 1023-1031.
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abstract = "Background: Alloplasts are inert foreign bodies acting as osteoconductive space maintainers during osseous wound healing. They may also function as carriers for growth factors that are known to enhance neovascularization and osteoinduction; human recombinant transforming growth factor beta (rhTGF-β1) is one such factor. The purpose of this study was to evaluate di-vinyl styrene beads (DVSb) and rhTGF-β1 effects on osseous regeneration in the rat calvaria critical-sized defect model. Methods: Di-vinyl styrene beads (DVSb) with and without rhTGF-β1 were placed between gelfilm membranes in a critical-sized defect in the rat (Rattus norvegicus) calvaria. Actual bone fill; percentage bone fill; bone formation rate (BFR); and mineralization at 3, 6, and 12 weeks postsurgery were measured using densitometry, histomorphometry, scanning electron microscopy (SEM), and vital staining with tetracycline-HCI. Results: Mean radiographic density and percentage fill were statistically greater for DVSb treatment groups as compared with controls (P ≤0.05). BFR was consistently between 3 and 7 μm per day for all groups; alloplast group BFR was significantly greater than controls or the rhTGF-β1 groups at 6 weeks (P ≤0.05); however, at 3 and 12 weeks, the control BFR was greater than treatment groups (P ≤0.05). Membranes often collapsed and little bone fill or mineralization occurred in defects without DVSb. Mineralization appeared to occur adjacent to the alloplast by 12 weeks in the histologic and SEM sections. While DVSb fibrous attachment occurred in some specimens, there was no evidence of an inflammatory response. Conclusion: Di-vinyl styrene beads, with or without rhTGF-β1, significantly enhance bone regeneration in the rat calvaria defect model.",
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AU - Billman, Michael A.

AU - Sharawy, Mohamed

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