Evaluating changes of blood flow in retina, choroid, and outer choroid in rats in response to elevated intraocular pressure by 1300 nm swept-source OCT

Jingjiang Xu, Yuandong Li, Shaozhen Song, William Cepurna, John Morrison, Ruikang K. Wang

Research output: Contribution to journalArticle

Abstract

We report the development of a 1300 nm swept-source optical coherence tomography (SS-OCT) system specifically designed to perform OCT imaging and optical microangiography (OMAG) in rat eyes in vivo and its use in evaluating the effects of intraocular pressure (IOP) elevation on ocular circulation. The swept laser is operated in single longitude mode with a 90 nm bandwidth centered at 1300 nm and 200 kHz A-line rate, providing remarkable sensitivity fall-off performance along the imaging depth, a larger field of view of 2.5 × 2.5 mm2 (approximately 35°), and more time-efficient imaging acquisition. The advantage of the SS-OCT/OMAG is highlighted by an increased imaging depth of the entire posterior thickness of optic nerve head (ONH) and its surrounding vascular anatomy, to include, for the first time in vivo, the vasculature at the scleral opening, allowing visualization of the circle of Zinn-Haller and posterior ciliary arteries (PCAs). Furthermore, the capillary-level resolution angiograms achieved at the retinal and choroidal layers over a larger field of view enable a significantly improved quantification of the response of vascular area density (VAD) to elevated IOP. The results indicate that reduction in perfusion of the choroid in response to elevated IOP is delayed compared to that seen in the retina; while choroidal VAD doesn't reach 50% of baseline until ~70 mmHg, the same effect is seen for the retinal VAD at ~60 mmHg. The superior image quality offered by SS-OCT may allow more comprehensive investigation of IOP-related ocular perfusion changes and their pathological roles in glaucomatous optic nerve damage.

LanguageEnglish (US)
Pages37-45
Number of pages9
JournalMicrovascular Research
Volume121
DOIs
StatePublished - Jan 1 2019

Fingerprint

Choroid
Intraocular Pressure
Retina
Rats
Optical tomography
Optical Coherence Tomography
Blood
Blood Vessels
Imaging techniques
Optics
Perfusion
Ciliary Arteries
Retinal Vessels
Optical Imaging
Optic Disk
Optic Nerve
Laser modes
Image quality
Anatomy
Angiography

Keywords

  • Circle of Zinn-Haller
  • Glaucoma
  • Intraocular pressure
  • Ocular perfusion
  • Optic nerve head
  • Optical coherence tomography
  • Optical microangiography
  • Swept source OCT

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

Cite this

Evaluating changes of blood flow in retina, choroid, and outer choroid in rats in response to elevated intraocular pressure by 1300 nm swept-source OCT. / Xu, Jingjiang; Li, Yuandong; Song, Shaozhen; Cepurna, William; Morrison, John; Wang, Ruikang K.

In: Microvascular Research, Vol. 121, 01.01.2019, p. 37-45.

Research output: Contribution to journalArticle

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