Estrogen receptor beta (ERβ) mRNA and protein in serotonin neurons of macaques

Chrisana Gundlah, Nick Z. Lu, Stephanie J. Mirkes, Cynthia Bethea

    Research output: Contribution to journalArticle

    101 Citations (Scopus)

    Abstract

    This study used double in situ hybridization (ISH) to examine the colocalization of estrogen receptor beta (ERβ) mRNA in serotonin neurons of rhesus macaques (Macaca mulatta). In addition, immunocytochemistry (ICC) was used to examine the expression and regulation of ERβ protein in raphe neurons of the macaque midbrain. For double ISH, monkey specific riboprobes for ERβ incorporating radiolabeled-UTP and a riboprobe for the human serotonin reuptake transporter (SERT) incorporating digoxigenin were applied to midbrain sections from spayed rhesus macaques. ERβ mRNA hybridization signal was expressed in most cells containing SERT mRNA in the dorsal and median raphe and pons. There were also non-SERT neurons expressing ERβ mRNA. In addition, ERβ protein was detected with an affinity purified polyclonal antibody generated against a synthetic peptide corresponding to the D domain of human ERβ conjugated to bovine serum albumin (provided by Dr. Philippa Saunders, MRC, Edinburgh). Midbrain sections containing the dorsal raphe from spayed rhesus macaques with and without hormone replacement therapy were processed for ERβ immunostaining. ERβ protein was detected at a similar intensity and in a similar number of cells in the dorsal raphe neurons in all treatment groups. Thus, the expression of ERβ protein in the dorsal raphe was consistent with the expression of ERβ mRNA. In conclusion, ERβ mRNA is expressed by serotonin neurons and it is translated to protein. ERβ protein, like ERβ mRNA, is detected at similar levels in the presence or absence of ovarian hormones.

    Original languageEnglish (US)
    Pages (from-to)14-22
    Number of pages9
    JournalMolecular Brain Research
    Volume91
    Issue number1-2
    DOIs
    StatePublished - Jul 13 2001

    Fingerprint

    Estrogen Receptor beta
    Macaca
    Serotonin
    Neurons
    Messenger RNA
    Macaca mulatta
    Mesencephalon
    Serotonin Plasma Membrane Transport Proteins
    estrophilin
    In Situ Hybridization
    Digoxigenin
    Uridine Triphosphate
    Pons
    Hormone Replacement Therapy
    Bovine Serum Albumin
    Haplorhini

    Keywords

    • Estrogen receptor
    • Hormone replacement therapy
    • Mood disorder
    • Ovarian steroid
    • Primate
    • Serotonin

    ASJC Scopus subject areas

    • Molecular Biology
    • Cellular and Molecular Neuroscience

    Cite this

    Estrogen receptor beta (ERβ) mRNA and protein in serotonin neurons of macaques. / Gundlah, Chrisana; Lu, Nick Z.; Mirkes, Stephanie J.; Bethea, Cynthia.

    In: Molecular Brain Research, Vol. 91, No. 1-2, 13.07.2001, p. 14-22.

    Research output: Contribution to journalArticle

    Gundlah, Chrisana ; Lu, Nick Z. ; Mirkes, Stephanie J. ; Bethea, Cynthia. / Estrogen receptor beta (ERβ) mRNA and protein in serotonin neurons of macaques. In: Molecular Brain Research. 2001 ; Vol. 91, No. 1-2. pp. 14-22.
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