Estrogen induces rapid translocation of estrogen receptor β, but not estrogen receptor α, to the neuronal plasma membrane

L. C. Sheldahl, R. A. Shapiro, D. N. Bryant, Ines Koerner, Daniel Dorsa

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Estrogen receptors can activate transcription in the nucleus, and activate rapid signal transduction cascades in the cytosol. Multiple reports identify estrogen receptors at the plasma membrane, while others document the dynamic responses of estrogen receptor to ligand binding. However, the function and identity of membrane estrogen receptors remain controversial. We have used confocal microscopy and cell fractionation on the murine hippocampus-derived HT22 cell line and rat primary cortical neurons transfected with estrogen receptor-green fluorescent protein constructs to address the membrane localization of these receptors. We observe translocation of estrogen receptor beta (β) to the plasma membrane 5 min after exposure to 17β-estradiol, whereas estrogen receptor alpha (α) localization remains unchanged. Membrane localization of estrogen receptor β is transient, selective for 17β-estradiol, and is not blocked by ICI182,780. Inhibition of the mitogen-activated protein kinase pathway does not block estrogen-mediated estrogen receptor β membrane translocation, and in fact prolongs membrane localization. These data suggest that while both estrogen receptor α and estrogen receptor β can be present at the neuronal membrane, their presence is differentially regulated.

Original languageEnglish (US)
Pages (from-to)751-761
Number of pages11
JournalNeuroscience
Volume153
Issue number3
DOIs
StatePublished - May 15 2008

Fingerprint

Estrogen Receptors
Estrogens
Cell Membrane
Membranes
Estradiol
Cell Fractionation
Estrogen Receptor beta
Estrogen Receptor alpha
Green Fluorescent Proteins
Mitogen-Activated Protein Kinases
Confocal Microscopy
Cytosol
Signal Transduction
Hippocampus
Ligands
Neurons
Cell Line

Keywords

  • hormone
  • HT22
  • MAPK
  • neuron
  • steroid
  • subcellular localization

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Estrogen induces rapid translocation of estrogen receptor β, but not estrogen receptor α, to the neuronal plasma membrane. / Sheldahl, L. C.; Shapiro, R. A.; Bryant, D. N.; Koerner, Ines; Dorsa, Daniel.

In: Neuroscience, Vol. 153, No. 3, 15.05.2008, p. 751-761.

Research output: Contribution to journalArticle

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AU - Dorsa, Daniel

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