Essential role for diacylglycerol in protein transport from the yeast Golgi complex

Brian G. Kearns, Todd P. McGee, Peter Mayinger, Alma Gedvilaite, Scott E. Phillips, Satoshi Kagiwada, Vytas A. Bankaitis

Research output: Contribution to journalArticle

217 Citations (Scopus)

Abstract

Yeast phosphatidylinositol transfer protein (Sec14p) is required for the production of secretory vesicles from the Golgi. This requirement can be relieved by inactivation of the cytosine 5'-diphosphate (CDP)-choline pathway for phosphatidylcholine biosynthesis, indicating that Sec14p is an essential component of a regulatory pathway linking phospholipid metabolism with an integral membrane protein related to inositol-5-phosphatases such as synaptojanin, a protein found in rat brain. Here we show that defects in Sac1p also relieve the requirement for Sec14p by altering phospholipid metabolism so as to expand the pool of diacylglycerol (DAG) in the Golgi. Moreover, although short-chain DAG improves secretory function in strains with a temperature-sensitive Sec14p, expression of diacylglycerol kinase from Escherichia coli further impairs it. The essential function of Sec14p may therefore be to maintain a sufficient pool of DAG in the Golgi to support the production of secretory vesicles.

Original languageEnglish (US)
Pages (from-to)101-105
Number of pages5
JournalNature
Volume387
Issue number6628
StatePublished - May 1 1997
Externally publishedYes

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Diglycerides
Golgi Apparatus
Protein Transport
Yeasts
Secretory Vesicles
Phospholipids
Diacylglycerol Kinase
Phospholipid Transfer Proteins
Fungal Proteins
Diphosphates
Cytosine
Choline
Phosphatidylcholines
Membrane Proteins
Escherichia coli
Temperature
Brain
Proteins

ASJC Scopus subject areas

  • General

Cite this

Kearns, B. G., McGee, T. P., Mayinger, P., Gedvilaite, A., Phillips, S. E., Kagiwada, S., & Bankaitis, V. A. (1997). Essential role for diacylglycerol in protein transport from the yeast Golgi complex. Nature, 387(6628), 101-105.

Essential role for diacylglycerol in protein transport from the yeast Golgi complex. / Kearns, Brian G.; McGee, Todd P.; Mayinger, Peter; Gedvilaite, Alma; Phillips, Scott E.; Kagiwada, Satoshi; Bankaitis, Vytas A.

In: Nature, Vol. 387, No. 6628, 01.05.1997, p. 101-105.

Research output: Contribution to journalArticle

Kearns, BG, McGee, TP, Mayinger, P, Gedvilaite, A, Phillips, SE, Kagiwada, S & Bankaitis, VA 1997, 'Essential role for diacylglycerol in protein transport from the yeast Golgi complex', Nature, vol. 387, no. 6628, pp. 101-105.
Kearns BG, McGee TP, Mayinger P, Gedvilaite A, Phillips SE, Kagiwada S et al. Essential role for diacylglycerol in protein transport from the yeast Golgi complex. Nature. 1997 May 1;387(6628):101-105.
Kearns, Brian G. ; McGee, Todd P. ; Mayinger, Peter ; Gedvilaite, Alma ; Phillips, Scott E. ; Kagiwada, Satoshi ; Bankaitis, Vytas A. / Essential role for diacylglycerol in protein transport from the yeast Golgi complex. In: Nature. 1997 ; Vol. 387, No. 6628. pp. 101-105.
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