Erythropoietin kinetics in rats: Generation and clearance

S. E. Steinberg, J. F. Garcia, G. R. Matzke, J. Mladenovic

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Detailed studies to analyze the early events of erythropoietin (Ep) secretion and clearance were performed in a rat model using a double antibody radioimmunoassay. Ep clearance was determined following intravenous injection of 1 mL of Ep-rich plasma, 1,080 mU/mL, obtained from phlebotomized rats. Analysis revealed a disappearance curve that conformed to a two-compartment model with an α half-life t 1/2 of 3.6 minutes and a β t 1/2 of 86 minutes. The volume of distribution was similar to the calculated plasma volume. In anephric animals, there was no change in the plasma clearance rate or the volume of distribution. Rapid Ep secretion was elicited by a single 15 mL/kg phlebotomy (hematocrit decrement 45% to 30%), so that levels reached 20 to 30 times baseline (524 ± 76 v 24 ± 7 mU/mL) at five hours, whereas they plateaued for at least 33 hours. The increase in the rate of secretion was geometric, from 9.9 mU/h baseline secretion to 429 mU/h. These data identify a very sensitive and rapidly responsive system for Ep modulation in the rat.

Original languageEnglish (US)
Pages (from-to)646-649
Number of pages4
JournalBlood
Volume67
Issue number3
DOIs
StatePublished - 1986

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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    Steinberg, S. E., Garcia, J. F., Matzke, G. R., & Mladenovic, J. (1986). Erythropoietin kinetics in rats: Generation and clearance. Blood, 67(3), 646-649. https://doi.org/10.1182/blood.v67.3.646.646