Novel single chain MHC class II constructs consisting of only the β1 and α1 domains were produced in E. coli covalently linked to an encephalitogenic peptide known to induce T cell mediated experimental autoimmune encephalomyelitis (EAE) in Lewis rats, or complexed with these peptides after purification. The β1α1/MBP-72-89 complex bound specifically to and inhibited activation of MBP-72-89 reactive T cells, and fully suppressed clinical and histological signs of EAE, blocking activation and recruitment of inflammatory T cells into the CNS. Moreover, this complex completely arrested progression of ongoing disease, inhibiting T cell activation in an IL-2 reversible manner. The potent biological properties of this new class of small soluble polypeptides provide a template for human homologues useful for detecting and deleting potentially pathogenic T cells specific for organ specific autoantigens.
|Original language||English (US)|
|State||Published - Mar 20 1998|
ASJC Scopus subject areas
- Molecular Biology