EPA, but not DHA, decreases mean platelet volume in normal subjects

Yongsoon Parka, William Harris

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The first indication of platelet activation is an increase in mean platelet volume (MPV). n-3 FA are known to inhibit platelet function and to reduce the risk for coronary heart disease. The purpose of this study was to determine the effects of EPA and DHA on MPV. Healthy subjects received olive oil placebo for 4 wk and then were randomly assigned to receive 4 g of ethyl esters of either safflower oil (n = 11), EPA (n = 10), or DHA (n = 12) for 4 wk. At the end of placebo run-in and treatment periods, MPV (fL; mean ± SEM) and platelet count (PLT-CT; 103/μL blood) were measured in the basal state and after ex vivo stimulation with collagen (10 μ/mL), cold (4°C), and heat (37°C). Unlike DHA, EPA lowered MPV as compared with safflower oil (7.2 ± 0.1 vs. 7.5 ± 0.1 fL; P <0.05) and raised PLT-CT (211 ± 18 vs.192 ± 18 103/μL; P <0.05) in the fasting state. Collagen and cold significantly increased MPV whereas heat lowered MPV regardless of treatment. All stimuli decreased PLT-CT. EPA significantly increased platelet EPA (0.2 ± 0.1 vs. 3.3 ± 0.4%) and docosapentaenoic acid (DPA; 2.2 ± 0.3 vs. 2.9 ± 0.3%) concentrations, but not DHA. DHA treatment significantly increased DHA (1.4 ± 0.2 vs. 4.1 ± 0.5%) and DPA (2.0 ± 0.4 vs. 3.0 ± 0.4%) concentrations, but not EPA. In conclusion, EPA, but not DHA, reduces platelet activation, an early step in platelet aggregation.

Original languageEnglish (US)
Pages (from-to)941-946
Number of pages6
JournalLipids
Volume37
Issue number10
DOIs
StatePublished - Oct 1 2002
Externally publishedYes

Fingerprint

Mean Platelet Volume
Platelets
Safflower Oil
platelet activation
safflower oil
Platelet Activation
placebos
collagen
Collagen
Blood Platelets
Hot Temperature
Placebos
docosapentaenoic acid
heat
blood platelet count
platelet aggregation
Platelet Count
Platelet Aggregation
olive oil
Coronary Disease

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Food Science
  • Biochemistry

Cite this

EPA, but not DHA, decreases mean platelet volume in normal subjects. / Parka, Yongsoon; Harris, William.

In: Lipids, Vol. 37, No. 10, 01.10.2002, p. 941-946.

Research output: Contribution to journalArticle

Parka, Yongsoon ; Harris, William. / EPA, but not DHA, decreases mean platelet volume in normal subjects. In: Lipids. 2002 ; Vol. 37, No. 10. pp. 941-946.
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abstract = "The first indication of platelet activation is an increase in mean platelet volume (MPV). n-3 FA are known to inhibit platelet function and to reduce the risk for coronary heart disease. The purpose of this study was to determine the effects of EPA and DHA on MPV. Healthy subjects received olive oil placebo for 4 wk and then were randomly assigned to receive 4 g of ethyl esters of either safflower oil (n = 11), EPA (n = 10), or DHA (n = 12) for 4 wk. At the end of placebo run-in and treatment periods, MPV (fL; mean ± SEM) and platelet count (PLT-CT; 103/μL blood) were measured in the basal state and after ex vivo stimulation with collagen (10 μ/mL), cold (4°C), and heat (37°C). Unlike DHA, EPA lowered MPV as compared with safflower oil (7.2 ± 0.1 vs. 7.5 ± 0.1 fL; P <0.05) and raised PLT-CT (211 ± 18 vs.192 ± 18 103/μL; P <0.05) in the fasting state. Collagen and cold significantly increased MPV whereas heat lowered MPV regardless of treatment. All stimuli decreased PLT-CT. EPA significantly increased platelet EPA (0.2 ± 0.1 vs. 3.3 ± 0.4{\%}) and docosapentaenoic acid (DPA; 2.2 ± 0.3 vs. 2.9 ± 0.3{\%}) concentrations, but not DHA. DHA treatment significantly increased DHA (1.4 ± 0.2 vs. 4.1 ± 0.5{\%}) and DPA (2.0 ± 0.4 vs. 3.0 ± 0.4{\%}) concentrations, but not EPA. In conclusion, EPA, but not DHA, reduces platelet activation, an early step in platelet aggregation.",
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