Enhanced pelvic responses to stressors in female CRF-overexpressing mice

M. Million, L. Wang, Mary Stenzel-Poore, S. C. Coste, P. Q. Yuan, C. Lamy, J. Rivier, T. Buffington, Y. Taché

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Acute stress affects gut functions through the activation of corticotropin-releasing factor (CRF) receptors. The impact of acute stress on pelvic viscera in the context of chronic stress is not well characterized. We investigated the colonic, urinary, and locomotor responses monitored as fecal pellet output (FPO), urine voiding, and ambulatory activity, respectively, in female and male CRF-overexpressing (CRF-OE) mice, a chronic stress model, and their wild-type littermates (WTL). Female CRF-OE mice, compared with WTL, had enhanced FPO to 2-min handling (150%) and 60-min novel environment (155%) but displayed a similar response to a 60-min partial restraint stress. Female CRF-OE mice, compared with WTL, also had a significantly increased number of urine spots (7.3 ± 1.4 vs. 1.3 ± 0.8 spots/h) and lower locomotor activity (246.8 ± 47.8 vs. 388.2 ± 31.9 entries/h) to a novel environment. Male CRF-OE mice and WTL both responded to a novel environment but failed to show differences between them in colonic and locomotor responses. Male WTL, compared with female WTL, had higher FPO (113%). In female CRF-OE mice, the CRF1/CRF2 receptor antagonist astressin B and the selective CRF2 receptor agonist mouse urocortin 2 (injected peripherally) prevented the enhanced defecation without affecting urine or locomotor responses to novel environment. RT-PCR showed that CRF1 and CRF2 receptors are expressed in the mouse colonic tissues. The data show that chronic stress, due to continuous central CRF overdrive, renders female CRF-OE mice to have enhanced pelvic and altered behavioral responses to superimposed mild stressors and that CRF1-initiated colonic response is counteracted by selective activation of CRF2 receptor.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume292
Issue number4
DOIs
StatePublished - Apr 2007

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Corticotropin-Releasing Hormone
Urine
Corticotropin-Releasing Hormone Receptors
Defecation
Viscera
Locomotion
Polymerase Chain Reaction
CRF receptor type 2

Keywords

  • Astressin B
  • Chronic stress
  • Colon
  • Locomotor activity
  • Mouse urocortin 2
  • Urine

ASJC Scopus subject areas

  • Physiology

Cite this

Enhanced pelvic responses to stressors in female CRF-overexpressing mice. / Million, M.; Wang, L.; Stenzel-Poore, Mary; Coste, S. C.; Yuan, P. Q.; Lamy, C.; Rivier, J.; Buffington, T.; Taché, Y.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 292, No. 4, 04.2007.

Research output: Contribution to journalArticle

Million, M. ; Wang, L. ; Stenzel-Poore, Mary ; Coste, S. C. ; Yuan, P. Q. ; Lamy, C. ; Rivier, J. ; Buffington, T. ; Taché, Y. / Enhanced pelvic responses to stressors in female CRF-overexpressing mice. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2007 ; Vol. 292, No. 4.
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