Endothelial nitric oxide synthase in placental villous tissue from normal, pre-eclamptic and intrauterine growth restricted pregnancies

Leslie Myatt, A. L W Eis, D. E. Brockman, I. A. Greer, F. Lyall

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

Nitric oxide (NO) regulates blood flow in the human placenta. As increased resistance to blood flow is seen in the fetal-placental vasculature in pregnancies complicated by pre-eclampsia and/or intrauterine growth restriction (IUGR), we examined expression of endothelial nitric oxide synthase (eNOS) in these placentas. Placental villous tissue sections were obtained from normotensive control (n = 5), IUGR alone (n = 5) or pre-eclamptic (with or without IUGR) (n = 9) patients, immunostained for eNOS and scored for localization, type (punctate or diffuse) and intensity of eNOS staining in syncytiotrophoblast and placental vessels. The significance of differences was calculated using the Mann-Whitney U-test. No differences in intensity or type of immunostaining in syncytiotrophoblast were seen. Placentas from patients with pre-eclampsia with or without IUGR had a significantly more basal distribution of eNOS in syncytiotrophoblast. eNOS immunostaining was absent in terminal villous capillary and faint in stem villous vessel endothelium of normal placentas, but was intense in the endothelium of both of these types of vessels in the IUGR and pre-eclampsia groups, with significantly greater staining seen in stem vessels of patients with IUGR alone. This increased eNOS expression and hence increased NO production in the fetal-placental vasculature may be an adaptive response to the increased resistance and poor perfusion in these pathological pregnancies.

Original languageEnglish (US)
Pages (from-to)167-172
Number of pages6
JournalHuman Reproduction
Volume12
Issue number1
StatePublished - Jan 1997
Externally publishedYes

Fingerprint

Nitric Oxide Synthase Type III
Pregnancy
Placenta
Trophoblasts
Growth
Pre-Eclampsia
Endothelium
Nitric Oxide
Staining and Labeling
Nonparametric Statistics
Perfusion

Keywords

  • Endothelium
  • Nitric oxide
  • Placenta
  • Pre-eclampsia
  • Trophoblast

ASJC Scopus subject areas

  • Physiology
  • Developmental Biology
  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Endothelial nitric oxide synthase in placental villous tissue from normal, pre-eclamptic and intrauterine growth restricted pregnancies. / Myatt, Leslie; Eis, A. L W; Brockman, D. E.; Greer, I. A.; Lyall, F.

In: Human Reproduction, Vol. 12, No. 1, 01.1997, p. 167-172.

Research output: Contribution to journalArticle

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