Endoplasmic reticulum protein quality control is determined by cooperative interactions between Hsp/c70 protein and the CHIP E3 ligase

Yoshihiro Matsumura, Juro Sakai, William R. Skach

    Research output: Contribution to journalArticlepeer-review

    36 Scopus citations

    Abstract

    Background: The CHIP E3 ligase regulates Hsp70 pro-degradation activities. Results: The P269A CHIP U-box mutation induces CHIP oligomerization and modulates nucleotide- and substrate-dependent interactions between the TPR domain and Hsp70 C terminus. Conclusion: The U-box domain plays a key role in CHIP recruitment to Hsp70-client complexes, possibly by controlling oligomerization. Significance: Hsp70-CHIP substrate triage is governed by complex allosteric interactions between multiple domains in both proteins.

    Original languageEnglish (US)
    Pages (from-to)31069-31079
    Number of pages11
    JournalJournal of Biological Chemistry
    Volume288
    Issue number43
    DOIs
    StatePublished - Oct 25 2013

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

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