Encephalitogenic T cell clones with variant receptor specificity

Halina Offner, G. A. Hashim, Y. K. Chou, B. Celnik, R. Jones, Arthur Vandenbark

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We explored antigenic differences between guinea pig (GP)-basic protein (BP), rat (Rt)-BP, and respective peptides from the encephalitogenic region for Lewis rats by comparing the fine specificity of T lymphocyte lines and clones selected from animals primed with these Ag. Encephalitogenic T cell lines specific for GP-BP or Rt-BP predictably recognized the corresponding 72-89 and to a lesser degree the 72-84 (S55S) amino acid sequence. T cell lines selected from rats primed with GP-S55S responded preferentially to GP-S55S compared to other peptides. A T cell line raised to Rt-S55S, however, initially recognized the S55S and S72-89 peptides but were nearly unresponsive to the intact GP-BP or Rt-BP. T cell clones selected from the Rt-S55S line at that point had two distinct patterns of response: clones that recognized both of the BP and the S55S peptides adoptively transferred delayed-type hypersensitivity and experimental autoimmune enchephalomyelitis. These clones also recognized residues 69-81 (S67) but not peptide S75-89. In contrast, T cell clones that responded only to synthetic peptides GP-S55S and Rt-S55S but not to the parent BP adoptively transferred delayed-type hypersensitivity but not disease in Lewis rats. The same clones failed to respond to either the S67 or the S75-89 sequences. These results demonstrate that the encephalitogenic Rt-S55S sequence houses a minimun of two T cell epitopes with differing specificities and functions. One epitope is immuno-dominant and resembles the encephalitogenic region of the intact BP molecule. The second non-encephalitogenic epitope is restricted to the S55S sequences and is not shared by the parent BP, the S67, or the S75-89 sequences. Both types of Rt-S55S-specific clones differ in fine specificity from encephalitogenic clones selected from GP-BP immunized rats, thus indicating that uniformity of T cell recognition of the encephalitogenic epitope is not an absolute condition for T cells to be encephalitogenic.

Original languageEnglish (US)
Pages (from-to)3828-3832
Number of pages5
JournalJournal of Immunology
Volume141
Issue number11
StatePublished - 1988

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Clone Cells
T-Lymphocytes
Guinea Pigs
2,2-dithiobis(N-(1-adamantyl)methyl)acetamide
Proteins
Peptides
Epitopes
Delayed Hypersensitivity
Cell Line
T-Lymphocyte Epitopes
Amino Acid Sequence

ASJC Scopus subject areas

  • Immunology

Cite this

Offner, H., Hashim, G. A., Chou, Y. K., Celnik, B., Jones, R., & Vandenbark, A. (1988). Encephalitogenic T cell clones with variant receptor specificity. Journal of Immunology, 141(11), 3828-3832.

Encephalitogenic T cell clones with variant receptor specificity. / Offner, Halina; Hashim, G. A.; Chou, Y. K.; Celnik, B.; Jones, R.; Vandenbark, Arthur.

In: Journal of Immunology, Vol. 141, No. 11, 1988, p. 3828-3832.

Research output: Contribution to journalArticle

Offner, H, Hashim, GA, Chou, YK, Celnik, B, Jones, R & Vandenbark, A 1988, 'Encephalitogenic T cell clones with variant receptor specificity', Journal of Immunology, vol. 141, no. 11, pp. 3828-3832.
Offner, Halina ; Hashim, G. A. ; Chou, Y. K. ; Celnik, B. ; Jones, R. ; Vandenbark, Arthur. / Encephalitogenic T cell clones with variant receptor specificity. In: Journal of Immunology. 1988 ; Vol. 141, No. 11. pp. 3828-3832.
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