Encapsidation of truncated human hepatitis b virus genomes through trans-complementation of the core protein and polymerase

Pei Wen Chiang, Cheng Po Hu, Tsung Sheng Su, Szecheng J. Lo, Ming Huey H. Chu, Heinz Schaller, Chungming Chang

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Mutational analyses and complementation tests were used to analyze the strategy of packaging and of replication of human hepatitis B virus (HBV). By creating new restriction enzyme sites and by varying the genome length of HBV mutants, we identified that the mutated genomes could be encapsidated through trans-complementation of the polymerase and/or core protein. This study demonstrates that the polymerase of HBV, similar to that of duck hepatitis B virus (DHBV), is synthesized de novo instead of through a core-polymerase fusion protein. The results also indicate that both the polymerase and the core protein can be supplied in trans during viral packaging, and that the complementation is not due to recombination between the cotransfected plasmids. Furthermore, HBV genome deleted down to 2.4 kb is still able to be encapsidated, as measured by the endogenous polymerase reaction. Taken together, these results provide a basis for using HBV as a vector to deliver foreign genes into hepatocytes and for defining the location of the packaging signal on the HBV genome.

Original languageEnglish (US)
Pages (from-to)355-361
Number of pages7
JournalVirology
Volume176
Issue number2
DOIs
StatePublished - Jun 1990

ASJC Scopus subject areas

  • Virology

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    Chiang, P. W., Hu, C. P., Su, T. S., Lo, S. J., Chu, M. H. H., Schaller, H., & Chang, C. (1990). Encapsidation of truncated human hepatitis b virus genomes through trans-complementation of the core protein and polymerase. Virology, 176(2), 355-361. https://doi.org/10.1016/0042-6822(90)90005-C